白薇提取液对意大利青霉抑菌机理及脐橙青霉病防治效果的研究  

Antifungal mechanism of Cynanchum atratum Bunge extracts on Penicillium italicum and control effect on blue mold of navel orange

作　　者：彭旋[1,2] 陈玉环[1,2] 陈金印[1,2] 

机构地区：[1]江西省果蔬采后处理关键技术与质量安全协同创新中心,江西农业大学农学院,南昌330045 [2]江西省果蔬保鲜与无损检测重点实验室,江西农业大学农学院,南昌330045

出　　处：《植物病理学报》2017年第3期398-405,共8页Acta Phytopathologica Sinica

基　　金：“十二五”国家科技支撑计划项目(2012BAD38B03-2);江西省教育厅科技落地计划项目(111);江西省“赣鄱英才555工程”(2012)

摘　　要：试验以意大利青霉(Penicillium italicum)为供试菌,研究不同白薇提取液浓度对意大利青霉孢子萌发率、菌丝体总糖和可溶性蛋白质含量、苹果酸脱氢酶和琥珀酸脱氢酶活性以及培养液电导率的影响,并观察测定白薇提取液对脐橙青霉病的防治效果。结果显示:白薇提取液能够抑制意大利青霉的孢子萌发,在浓度为50.00 mg·mL-1时,孢子萌发的抑制率高达95.43%,此外,白薇提取液还能增强菌丝体细胞膜渗透性,降低菌丝体总糖和可溶性蛋白质含量以及苹果酸脱氢酶和琥珀酸脱氢酶活性,显著降低果实病斑直径。白薇提取液主要是抑制意大利青霉孢子的萌发、破坏细胞膜结构以及降低能量代谢酶活性,从而扰乱意大利青霉正常生长,减少脐橙青霉病的发生。Penicillium italicum was used to evaluated the effects of Cynanchum atraum Bunge extracts with spore germination, content of total sugar, soluble protein, activity of malatedehydrogenase and succinatede- hydrogenase, and electric conductivity of broth for P. italicurn as well as the control effect on postharvest blue mold of navel orange. The result showed that the C. atratum extracts could inhibit the spore germination of P. italicum at a rate of even 95.43% when 50.00 mg .mL1 of extract was applied. Additionally, C. atratum extracts could enhance the cytomembrane permeability of mycelium and the content of total carbohydrate and soluble protein. Moreover, the activity of malatedehydrogenase and succinatedehydrogenase was decreased and the diameter of spots on fruit was depressed significantly. In summary, C. atratum extracts could disturb the growth of P. italicum and reduced the occurrence of navel orange penicilliosis through mainly inhibition of spore germination, destroying the structure of cytomenbrane, and decreasing the activity of energy metabolism enzyme.

关 键 词：白薇 脐橙 青霉病 抑菌机理 防治效果 

分 类 号：S436.661[农业科学—农业昆虫与害虫防治]