转AO基因高植酸酶玉米营养学评价分析  

Nutritional Evaluation on AO Transgenic High Phytase Maize

作　　者：张宇[1] 令狐丽琴 胡贻椿[1] 杨晓光[1] 朴建华[1] 张一平[1] 杨丽琛[1] ZHANG Yu LINGHU Li-qin HU Yi-chun YANG Xiao-guang PIAO Jian-hua ZHANG Yi-ping YANG Li-chen(National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention/ Key Laboratory of Trace Element Nutrition, National Health and Family Planning Commission, Beijing 100050, China Shanxi Center for Disease Control and Prevention, Taiyuan 030012, China)

机构地区：[1]中国疾病预防控制中心营养与健康所/卫生部微量元素营养重点实验室,北京100050 [2]山西省疾病预防控制中心,太原030012

出　　处：《中国食物与营养》2017年第5期50-54,共5页Food and Nutrition in China

基　　金：转基因生物新品种培育科技重大专项"优质功能型转基因玉米新品种培育"(项目编号:2014ZX08003-002)

摘　　要：目的:评价转AO基因高植酸酶玉米的主要营养学价值。方法:以国标法测定转基因玉米及其亲本的主要营养成分。选择断乳SD大鼠100只随机分为5组,饲养28d。对5组大鼠饲养前、后主要营养学指标、血液和生化学指标,以及蛋白质功效比值(PER)进行比较。结果:转AO基因高植酸酶玉米与非转基因对照玉米在主要营养成分方面均有相似性。各试验组大鼠的平均增重和平均日增重量均显著高于对照组(P<0.05),且A组的平均增重亦显著高于B组(P<0.05)。雄性试验组和雌性A、B组的WBC计数显著低于对照组(P<0.05),雄性B、D组的AST,以及A组的CRE均显著高于对照组(P<0.05),雄性C组的AST显著低于D组(P<0.05)。所有试验组的PER均显著低于对照组(P<0.05),转基因玉米与对照玉米在PER和校正PER方面均无统计学差异(P>0.05)。结论:转AO基因高植酸酶玉米在蛋白质营养价值方面与非转基因玉米具有实质等同性,从营养学角度分析该类型玉米是安全的。Objective To evaluate the nutrition of AO transgenic high phytase maize.Method National standard method was used to determine the main nutrients in both genetically modified and parental corn.100 weaning SD rats divided into 5 groups and feeding for 28days,and the nutritional,hematological and biochemical indicators before and after test,and also the protein efficacy ratio were compared.Result The main nutrients in both genetically modified and parental corn were similar.The average weight gain and average daily gain of test groups were all lower than control group（P〈0.05）,and average daily gain of group A was higher than group B（P〈0.05）.The white blood cell count both of male rats,and female rates in group A and B were all lower than control group（P〈0.05）,AST of male rats in group B and D,CRE of group A were all higher than control group（P〈0.05）,and the AST of male rats in group C were lower than group D（P〈0.05）.The PER of all the test groups were lower than control group（P〈0.05）,and the PER and corrected PER of genetically modified and parental corn were no difference（P〉0.05）.Conclusion The AO transgenic high phytase maize has substantial equivalence as its non-GM maize,and this type maize is safe from the view of nutritional point.

关 键 词：转基因玉米 植酸酶 28d喂养试验 蛋白质功效评价 营养成分分析 

分 类 号：TS201.4[轻工技术与工程—食品科学]