甲状腺乳头状癌中D2-40、EGFR和VEGF的表达及BRAF基因突变  

Clinical significance of D2-40,EGFR and VEGF expression and BRAF mutation in papillary thyroid carcinoma

作　　者：巩雷[1] 陈萍[1] 刘宪军[1] 刘学鑫 Gong Lei Chen Ping Liu Xianjun et al(Department of Pathology,Jilin Central General Hospital,Jilin 132011 ,China)

机构地区：[1]吉林市中心医院病理科,吉林吉林132011

出　　处：《实用肿瘤杂志》2017年第3期234-239,共6页Journal of Practical Oncology

摘　　要：目的探讨甲状腺乳头状癌中D2-40、表皮生长因子受体(epidermal growth factor receptor,EGFR)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达与鼠类肉瘤滤过性毒菌致癌同源体B1(v-raf murine sarcoma viral oncogene homolog B1,BRAF)基因突变及其临床意义。方法选取87例甲状腺乳头状癌和48例甲状腺乳头状增生手术切除标本,应用扩增阻碍突变系统(amplification refractory mutation system,ARMS)及免疫组织化学法检测甲状腺乳头状癌和乳头状增生中BRAF基因突变情况和D2-40、EGFR及VEGF蛋白表达情况。结果甲状腺乳头状癌中BRAF基因突变率与D2-40、EGFR和VEGF蛋白阳性表达率均高于甲状腺乳头状增生(均P<0.05)。甲状腺乳头状癌有淋巴结转移组BRAF基因突变率和D2-40、EGFR及VEGF蛋白阳性表达率均高于无淋巴结转移组(均P<0.05)。结论 BRAF突变检测联合D2-40、EGFR及VEGF检测可以提高甲状腺乳头状癌的确诊率,并可能是预测甲状腺乳头状癌淋巴结转移与否的重要指标。Objective To investigate the clinical significance of the expression of D2-40, epidermal growth factor receptor （EGFR） and vascular endothelial growth factor （VEGF）, as well as the rate of v-raf murine sareome viral onco- gene homolog B1 （BRAF） gene mutation in papillary thyroid carcinoma. Methods Surgical specimens of 87 cases of pa- pillary thyroid carcinoma and 48 cases of thyroid papillary hyperplasia were included. BRAF gene mutation rate and the protein levels of D2-40, EGFR and VEGF were examined by amplification refractory mutation system （ARMS） and immu- nohistochemical staining SP. Results Both the BRAF mutation rate and the positive expression rates of D2-40, EGFR and VEGF proteins are significantly higher in papillary thyroid carcinoma than thyroid papillary hyperplasia （ P 〈 0.05 ）. More- over, compared with papillary thyroid carcinoma without lymph node metastasis, those cases with lymph node metastasis have significantly higher BRAF gene mutation rate and positive expression rates of D2-40, EGFR and VEGF （ P 〈 0.05 ）. Conclusion Joint detection of BRAF mutation and the expression of D2-40, EFGR and VEGF can improve the diagnostic rate of papillary thyroid carcinoma, and may be an important indicator for lymph node metastasis.

关 键 词：甲状腺肿瘤/病理学 甲状腺肿瘤/遗传学 癌 乳头状 基因 ERBB-1 受体 血管内皮生长因子 原癌基因蛋白质B-Raf 基因扩增 突变 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤] R730.23[医药卫生—临床医学]