离子注入选育高效石油烃降解菌研究  

Study on Screening of a Highly Petroleum Hydrocarbon-Degrading Bacteriaby Ion Implantation

作　　者：马睿[1] 蔡长龙[1] 梁海锋[1] 严一心[1] MA Rui CAI Changlong LIANG Hai feng YAN Yixin(Research Center of Ion Beam Bio-Engineering and Biodiversity, Xi ＇an Technological University, Xi ~ an 7 ] 0021, China)

机构地区：[1]西安工业大学离子束生物工程与生物多样性研究中心,西安710021

出　　处：《西安工业大学学报》2017年第2期129-133,共5页Journal of Xi’an Technological University

基　　金：陕西省教育厅专项科研计划项目(14JK1352);陕西省科学技术研究发展计划项目(2012K08-05)

摘　　要：为了提高不动杆菌对石油烃的降解能力,采用离子诱变育种技术,使用生物改性离子注入设备对不动杆菌诱变育种.通过研究不动杆菌a8在不同剂量N+离子注入后存活率的变化趋势,结合气相色谱法(GC)测定得到的N+离子注入前后不动杆菌对石油烃的降解率,获得了离子注入不动杆菌诱变育种的最佳工艺参数.研究结果表明:N^+离子注入剂量与不动杆菌存活率之间呈现出较明显的"马鞍形"变化趋势;离子注入后不动杆菌对石油烃的降解率均有所提高,降解率最高可达95.03%;N^+离子的注入能量和注入剂量分别为15keV和8.0×10^(15) ions·cm^(-2)是不动杆菌离子诱变的最佳工艺参数.To improve the ability of petroleum hydrocarbon-degrading, the acinetobacter was induced mutation by the biological modified ion implantation equipment. The variation of the survival rate of acinetobacter a8 after different doses of N^＋ ion implantation was researched,and the degradation rates of petroleum hydrocarbon was measured by gas chromatograph （GC）. The optimum implantation parameters of the acinetobacter a8 were determined. The results showed that there was a significant ＂ saddle-shaped＂ trend between N^6 ion implantation dose and acinetobacter survival rate. The degradation rate of petroleum hydrocarbon increased as high as 95.03% after ion implantation. The injection energy and implantation dose of N＋ ion were 15 keV and 8.0×10^15 ions·cm^-2,respectively,which are the optimum process parameters of acinetobacter ionization mutagenesis.

关 键 词：离子注入 不动杆菌 存活率 降解率 

分 类 号：X172[环境科学与工程—环境科学]