七彩菊花青素的沉淀法辅助提纯及其结构鉴定  

Precipitation auxiliary purification and structure identification of anthocyanins in Helichrysum bracteatum

作　　者：王琼[1] 邱昊旻 邓智剑 徐鹏[3] 李强[1,3] WANG Qiong QIU Hao-min DENG Zhi-jian XU Peng LI Qiang(College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083 The Affiliated International School of Shenzhen University, Shenzhen 518000 College of Science, Beijing Forestry University, Beijing 100083)

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]深大师范学院国际高中,深圳518000 [3]北京林业大学理学院,北京100083

出　　处：《中国食品添加剂》2017年第5期175-182,共8页China Food Additives

基　　金：中央高校基本科研业务费专项资金资助(2015ZCQ-LY-03);国家自然科学基金资助项目(21671021)

摘　　要：采用溶液浸泡法提取七彩菊花瓣中的花青素,提取溶剂为含1%盐酸的甲醇溶液。然后,与饱和醋酸铅溶液形成沉淀,并用含5%盐酸的甲醇溶液洗脱,对提取的花青素进行粗步纯化。经真空冷冻干燥,得花青素固体样品。重量法测定,七彩菊花瓣中的花青素含量约为6.87%。纸色谱(PC)分离主要有红色物质和黄色物质,通过颜色反应、紫外可见光谱、红外光谱的测定分析,认为红色物质为花青素,黄色物质为黄酮。液相色谱(HPLC)分离色素,主要含有4个组分。通过对比提纯样品和粗提纯样品液相色谱图,认为金属盐沉淀法对花青素提纯有效果。色素经液相色谱-质谱(LC-MS)联用仪的分离测定和结构分析,结果表明七彩菊花色素中主要含有矢车菊素,可能含有微量飞燕草素、牵牛花色素、天竺葵色素,芍药色素。The pigments from Helichrysum bracteatum were first extracted by 1% hydrochloric acid methanol, and then precipitated by saturated lead acetate solution, 5% hydrochloric acid methanol solution was used to preliminarily purified the extract. After vacuum freeze dried, solid anthocyanin was obtained. The content of anthocyanin in Helichrysum Bracteatum was about 6.87% by weight method. Through paper chromatography separation, the pigment contained red and yellow substances, and were confirmed as anthocyanin and flavone by ultraviolet visible spectrum and infrared spectrum analysis. After pigments were separated by HPLC, four components were found. Apparently, by comparing liquid chromatograms of the purified samples and crude materials, we find that this metal salt precipitation was an effective method for anthocyanin purification. Finally, the pigments were separated and structurally analyzed by Liquid Chromatography-Mass Spectrometry （LC-MS） . The results showed that cyanidin was the main bracteatum flower pigment mainly contains cornflower glucoside and its derivatives, traces of component, with a little amount of Helichrysum delphinidin, petunidin, pelargonidin and peonidin.

关 键 词：七彩菊 花青素 金属沉淀法 提纯 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]