小鼠肝组织扫描电镜样本的制备及改良方法  

Preparation of specimen about mouse liver tissue for scanning electron microscope and its improvement method

作　　者：姚莹华[1] 陈育尧[2] 吕志平[2] 

机构地区：[1]广州中医药大学附属汕头中医院科教科,广东汕头515031 [2]南方医科大学中医药学院,广东广州510515

出　　处：《中国医学装备》2017年第6期165-167,共3页China Medical Equipment

基　　金：国家自然科学基金(30973699)"肝窦内皮细胞的调节机制与肝主疏泄的相关性研究"

摘　　要：目的:探讨优化小鼠肝组织扫描电镜样本的制备方法。方法:通过改良灌注固定的部位、优化灌注材料及步骤、采用经门静脉灌注固定和改变固定流程等制备小鼠肝脏扫描电镜样本,并对比改良制备方法与常规制备方法的组织细胞形态观察结果。结果:改良后的样本制备方法较好地保存了小鼠肝组织以及细胞的生活状态,可清晰观察肝脏组织及细胞的超微形态结构,获得了颇为理想的观察效果,且操作简便,成功率高,优于传统制备方法。结论:经门静脉灌注固定制备小鼠扫描电镜样本是比较优越的扫描电镜制样方法,具有一定应用价值。Objective： To explore and optimize the preparation method of specimen about mouse liver tissue for scanning electron microscopy. Methods： The specimens of mouse liver using for scanning electron microscope were prepared through improving the fixed location of perfusion, optimizing material and procedure of perfusion, adopting fixation of perfusion via portal vein and changing the process of fixation and so on. And the results of morphological observation of histocyte were compared between the improved method and traditional method. Results： The improved preparation method could save the liver tissue and the living state of the cells in preferably situation. By using this method, the ultra-micro morphological structure of liver tissue and cell could be clearly observed and the ideal effect of observation could be achieved. Besides, its operation was simple and its success rate was high. Therefore, it was superior to traditional method. Conclusion： The preparation of specimen about the fixation of mouse liver tissue via perfusion of portal vein for scanning electron microscope is a superior method, which has certain applied value.

关 键 词：肝组织 扫描电镜 样本制备 改良方法 

分 类 号：R-332[医药卫生]