间歇式超声辅助金针菇菇根多糖提取工艺研究  

Study on Optimization of Intermittent Ultrasonic Assisted Extraction of Polysaccharide from Base of Flammulina velutipes Root

作　　者：张明[1] 李新胜[1] 马超[1] 王朝川[1] 孟晓峰[1] 

机构地区：[1]中华全国供销合作总社济南果品研究院,山东济南250014

出　　处：《食品研究与开发》2017年第11期39-44,共6页Food Research and Development

基　　金：泰山产业领军人才项目(LJNY2015004)

摘　　要：采用间歇式超声辅助提取金针菇菇根多糖,通过单因素试验和响应面试验设计优化金针菇菇根多糖提取工艺条件。结果表明,影响金针菇菇根多糖得率的工艺因素按主次顺序排列为后段超声时间(C)>恒温提取时间(B)>前段超声时间(A)。确定金针菇菇根多糖最佳提取工艺条件为提取液p H5,料液比1∶30(g/m L),提取温度90℃,超声功率400 W,前段超声时间20 min,恒温提取时间130 min,后段超声时间23 min,在此最佳条件下,多糖得率为7.24%。在超声时间和恒温提取时间相同条件下,该方式多糖得率比"前超声辅提+恒温提取"方式和"恒温提取+后超声辅提"方式分别提高6.3%和5.6%,比直接恒温水浴提取提高12.76%。Polysaccharide in base of Flammulina velutipes root was extracted with the assistance of intermittent ultrasonic. By single factor experiment and response surface design in optimizing the Flammulina velutipes root polysaccharides extraction conditions. The results showed, polysaccharide yield was most significantly affected by extraction after ultrasonic time, followed by thermostat extraction time and front ultrasonic time. An optimum polysaccharide yield of 7.24 % （on the basis of Flammulina velutipes root powder）were obtained when F/am- mulina velutipes root was treated with pH5, solid-liquid ratio 1 ： 30 （g/mL）, extraction temperature 90 ℃, ul- trasonic power 400 W, front ultrasonic time 20 rain, thermostat extraction time 130 min, after ultrasonic time 23 min. In the ultrasonic extraction time and thermostat extraction time under the same conditions,the polysac charide yield of the mode respectively 6.3 %, 5.6 %, 12.76 % than the ＂front ultrasound ＋ thermostat extraction＂ mode,the ＂thermostat extraction ＋ after ultrasound＂ mode, the ＂direct thermostatic water bath extraction＇mode.

关 键 词：金针菇菇根 多糖 间歇式超声辅提 响应面 

分 类 号：TQ464.1[化学工程—制药化工]