不同加工方法对延胡索3种生物碱含量的影响  

Effects of Different Processes on Contents of Three Alkaloid Ingredients in Coridalis Rhizoma

作　　者：龙全江[1] 张颖[1] 徐雪琴[1] 

机构地区：[1]安徽中医药高等专科学校,安徽芜湖241000

出　　处：《中国中医药信息杂志》2017年第7期90-93,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：安徽省高等教育振兴计划人才项目-省级专业带头人资助项目(2013年);芜湖市科技计划产学研合作重点项目(2013cxy03)

摘　　要：目的比较不同加工方法对延胡索药材中有效成分的影响。方法采用HPLC测定延胡索中原阿片碱、去氢紫堇碱、延胡索乙素的含量。色谱柱:Agilent HC-C18(2)柱(4.6 mm×250 mm,5μm);流动相:乙腈∶0.1%磷酸水溶液(三乙胺调p H 5.3)=26∶74;流速:1.0 m L/min;柱温:35℃;检测波长:280 nm;进样量:10μL。在上述色谱条件下,3种成分均得到很好分离,各成分质量与峰面积在相应范围内线性关系良好(r2≥0.999 6),平均加样回收率分别为100.31%、99.34%、99.34%(RSD≤2.70%)。结果大号规格(鲜品直径2.0~3.0 cm)煮制时间5~6 min、中号规格(鲜品直径1.5~1.9 cm)煮制时间2~4 min、小号规格(鲜品直径0.7~1.4 cm)煮制时间1~2 min者中原阿片碱、去氢紫堇碱、延胡索乙素的含量及3种生物碱总含量比各组其他样品高,经煮制加工的延胡索药材样品中3种生物碱的含量总体低于蒸制加工所得的延胡索药材。结论本研究为延胡索药材产地加工工艺的确定提供了依据。Objective To explore the effects of different processes on the contents of three alkaloid ingredients in Coridalis Rhizoma. Methods The contents of three alkaloid ingredients （protopine, dehydrocorydaline, tetrahydropalmatine） were determined by HPLC. Agilent HC-C18（2） column （4.6 mm × 250 mm, 5 μm） was set at 35 ℃; acetonitrile-0.1% phosphoric acid solution （triethylamine adjusted pH 5.3） （26：74） was set as mobile phase; the flow rate was 1.0 mL/min and detection wavelength was set at 280 nm; the injection volume was 10 μL. The three components were separated well under these chromatographic conditions. The linear relationship between the mass of each component and the peak area was good （r2≥0.999 6）. The average recoveries were 100.31%, 99.34%, and 99.34%, respectively （RSD≤2.70%）. Results The contents of protopine, dehydrocorydaline, tetrahydropalmatine and the total contents of three alkaloids were higher than other groups in large size samples （diameter of fresh products was 2.0–3.0 cm） which were boiled 5–6 minutes, the medium size samples （diameter of fresh products was 1.5–1.9 cm） which were boiled 2–4 minutes and the small size samples （diameter of fresh products was 0.7–1.4 cm） which were boiled 1–2 minutes. The contents of alkaloid in boiled process were lower than steamed process. Conclusion The results of study provide an experimental basis for origin processing of Coridalis Rhizoma.

关 键 词：延胡索 蒸制 煮制 生物碱 高效液相色谱法 

分 类 号：R282.4[医药卫生—中药学]