鲶鱼体表黏液粗提物抗菌作用活性及抑菌机理  

The Antimicrobial Effect and the Action Mechanism of Catfish Epidermal Mucus Extracts

作　　者：李婷婷 杨兵[2] 张慧芳[2] 陈颖[3] 励建荣[2] 

机构地区：[1]大连民族大学生命科学学院,辽宁大连116600 [2]渤海大学食品科学研究院辽宁省食品安全重点实验室,辽宁锦州121013 [3]中国检验检疫科学研究院,北京100123

出　　处：《中国食品学报》2017年第4期21-29,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31301572;31471639);中国博士后科学基金项目(2014M552302);"十二五"国家科技支撑计划(2012BAD29B06);重庆市博士后研究人员科研项目特别资助项目(Xm2014041)

摘　　要：采用琼脂扩散法测定鲶鱼体表黏液提取物(CEME)的抑菌活性;以腐败希瓦氏菌为研究对象,探讨CEME的抑菌机理。研究结果表明:CEME对革兰氏阳性菌和革兰氏阴性菌均有抑菌活性,具有广谱抗菌性,最小抑菌浓度在0.078~0.625 mg/m L;CEME能有效减缓腐败希瓦氏菌的生长速率;使用扫描电镜发现CEME对腐败希瓦氏菌的细胞形态结构造成明显的损伤,有效破坏微生物细胞膜的完整性;同时CEME还导致腐败希瓦氏菌细胞膜的通透性增加,从而使体内小分子物质以及部分大分子物质向外泄漏;应用SDS-PAGE方法发现CEME处理对腐败希瓦氏菌的总蛋白和细菌膜蛋白均有一定的影响,它能够抑制菌体某些蛋白的合成,导致胞内蛋白含量下降和缺失。In this study, the agar diffusion assay was utilized to investigate the antibacterial activities, and the action mechanism of CEME against Shewanella putrefaciens was detected. The data from minimum inhibitory concentration （MIC） values showed that CEME effectively inhibited the growth of all tested bacterial pathogens, and the MIC values were ranging from 0.078 to 0.625 mg/mL. CEME morphologies of bacteria treated with CEME were investigated by scan- ning electron microscopy （SEM）, which indicated that the primary inhibitory action of CEME was to damage the bacteri- al cell membranes. CEME also increased the permeability of the outer and inner membranes of Shewanella putrefaciens and disrupted the cell membrane with the release of small cellular molecules. As a result, CEME resulted in the loss of the barrier function and induced slight leakage of nucleotide. SDS-PAGE method showed that CEME treatment had a certain effect on total protein and membrane proteins of ShewaneUa putrefaciens, which could inhibit the synthesis of certain protein, therefore resulted in the decline and loss of intracellular protein content.

关 键 词：鲶鱼体表黏液提取物 腐败希瓦氏菌 抗菌活性 作用机理 

分 类 号：TS202.3[轻工技术与工程—食品科学]