元素分析/液相色谱-碳同位素比值质谱法在纯正苹果汁掺假鉴别中的应用  

Application of elemental analysis/liquid chromatography-isotope ratio mass spectrometry in detection of pure apple juice adulteration

作　　者：谭梦茹 张睿[2] 费晓庆[2] 吴斌[2] 沈崇钰[2] 丁涛[2] 刘芸[2] 

机构地区：[1]无锡市药品安全检验检测中心,无锡214000 [2]江苏出入境检验检疫局食品实验室,南京210001

出　　处：《食品安全质量检测学报》2017年第5期1565-1571,共7页Journal of Food Safety and Quality

基　　金：江苏出入境检验检疫局科技计划项目(2015KJ31)~~

摘　　要：目的建立一种鉴别检测苹果汁掺假情况的元素分析/液相色谱-同位素比值质谱法(elemental analysis/liquid chromatography-isotope ratio mass spectrometry,EA/LC-IRMS)。方法通过对不同产区不同种类118个纯正苹果汁的糖、有机酸、果糖、葡萄糖、二糖、寡糖碳同位素比值(δ^(13)C值)的测定,建立纯正苹果汁同位素数据库进而提出纯正苹果汁应满足的δ^(13)C值要求。结果有机酸和糖差值Δδ^(13)CO-S在-1.38‰至1.09‰范围内,果糖和葡萄糖差值Δδ^(13)CF-G在-0.70‰至0.57‰范围内,而各组分最大差值Δδ^(13)Cmax<4.36‰。对市售105个苹果汁进行检测,采用本方法检出32个阳性样品,而采用本实验室的糖浆标志物法仅检出12个阳性样品。结论本方法大大提高了苹果汁的掺假鉴别,有很大的实际应用潜力。Objective To establish a method for adulteration identification of apple juice by elemental analysis/liquid chromatography-isotope ratio mass spectrometry （EA/LC-IRMS）.Methods Based on the individual δ^13C values of sugar,organic acid,fructose,glucose,disaccharide,oligosaccharide carbon isotope ratios collected for 118 pure apple juices from different producing areas with different varieties,the pure apple juice isotope database was established and the δ^13C value limits for the pure apple juice were proposed.Results The δ^13C difference between organic acid and sugar （△δ^13Co-s） was-1.38 ‰o～1.09 ‰,δ^13C difference between fructose and glucose （△δ^13CF-G） was-0.70 ‰～0.57 ‰,and the maximum difference between all δ^13C values （△δ^13Cmax） should be less than 4.36 ‰.Under the pure apple juice criteria,LC/EA-IEMS method confirmed 32 positive samples,while the syrup marker method only detected 12 samples among 105 commercial samples.Conclusion The novel developed method represents a significant improvement,which has good potential in practical application.

关 键 词：元素分析 液相色谱 稳定同位素质谱 苹果汁掺假 

分 类 号：O657.63[理学—分析化学] TS255.44[理学—化学]