microRNA-34a和Notch1信号通路在颞叶癫痫模型大鼠海马中的表达及作用机制  

Expression levels and mechanism of microRNA -34a and Notchl signaling pathway in hippocampi of temporal lobe epilepsy rats

作　　者：姚宝珍[1] 黄婷婷[1] 袁昊[1] 余诗倩 张海菊[1] 

机构地区：[1]武汉大学人民医院儿科,430060

出　　处：《中华实用儿科临床杂志》2017年第12期932-935,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：湖北省卫计委基金项目（JX6865）

摘　　要：目的探讨mieroRNA（miR）-34a和Notehl信号通路在颞叶癫痫模型大鼠海马中的表达及作用机制。方法将大鼠根据随机数字表法分为模型组（40只）和对照组（40只），模型组采用腹腔注射氯化锂-匹罗卡品建立颞叶癫痫模型；对照组注射等量9g／L盐水。在建模后24h、3d、7d、15d、1个月采用Racine分级法评估大鼠行为学改变；实时荧光定量聚合酶链式反应检测海马中miR-34a及NotehlmRNA的表达水平；Westernblot检测Notchl蛋白的表达水平。结果模型组miR-34a24h、3d、7d、15d时表达水平分别为2．55±0．29、2．11±0．17、1．68±0．49、1．84±0．42，与对照组（1．00±0．00）比较差异均有统计学意义（t=-1．55、-1．11、-0．68、-0．84，均P〈0．05）。模型组Notchl mRNA24h、3d、7d、15d、1个月时表达水平分别为1．44±0．31、1．27±0．13、1．52±0．28、0．91±0．33、0．80±0．09，24h和7d表达水平与对照组（1．00±0．00）比较差异均有统计学意义（t=-0．44、-0．52，均P〈0．05）。模型组15d时NotehlmRNA表达水平较24h和7d降低（t=-0．54、-0．62）；1个月时表达水平较24h、3d、7d降低（t=-0．64、-0．46、-0．72），差异均有统计学意义（均P〈0．05）。模型组Notchl蛋白24h、3d、7d、15d、1个月时表达水平分别为0．78±0．09、0．57±0．13、0．55±0．16、0．42±0．13、0．33±0．09，24h时表达水平与对照组（0．51±0．15）比较差异有统计学意义（t=-0．20，P〈0．05）。模型组Notchl蛋白15d时表达水平较24h降低（t=-0．26，P〈0．05）；1个月时表达水平较24h、3d降低（t=-0．36、-0．24），差异均有统计学意义（均P〈0．05）。结论颞叶癫痫大鼠模型海马中miR-34a表达水平上调，可能通过激活Notehl信号通路参与癫痫的发生与发展。Objective To explore the expression levels of microRNA（miR） -34a in hippocampus of temporal lobe epilepsy rats and the effect of miR - 34a on its target signaling pathway Notehl. Methods Rats were divided randondy into experiment group （ n = 40 ） and control group （ a = 40 ） by adopting random nmnber table method. The status epilepticus model and the temporal lobe epilepsy model were induced by using lithium - pilocarpine for experiment group. Tile control group rats received an injection of an equal amount of 9 g/L saline as instead of pilocarpine. Racine grading was performed at 24 hours,day 3, day 7, （lay 15, and 1 month after modeling to evaluate the behavior. Real - time fluorescent quantitative polymerase chain reaction was used to test the mRNA expressions of miR -34a and Notchl. Western blot was performed to explore the protein expression of Notehl. Results The expression levels of miR - 34a at post - status cpilcptieus in 24 hours,day 3, day 7, day 15 were 2.55 ± 0.29,2. 1 l ± 0. 17,1.68 ± 0.49 and 1.84 ＋ 0. 42, respectively, which showed statistically significant difference compared with the control group （ 1.00 ±0.00） （t = - 1.55, - 1.11, -0.68, -0.84,all P 〈0.05）. The expression levels of Notchl mRNA at post - status epilepticus in 24 hours,day 3, day 7, day 15,1 month were 1.44 ＋_ 0.31,1.27 ＋__ 0.13,1.52 ＋_ 0.28,0.91 ＋__ 0.33,and 0.80 ± 0.09 respectively. There were significant differences at 24 hours, day 7 in Notehl mRNA expression （ t = - 0.44, - 0.52, all P 〈 0.05 ） compared with the control group （ 1.00 ± 0.00 ）. The expression level of Notehl mRNA on day 15 was significantly lower than 24 hours and day 7 （ t = - 0.54, - 0.62, all P 〈 0.05 ） ,and the expression in 1 month was significantly lower than in 24 hours, or day 3 and day 7 （ t = - 0.64, - 0.46, - 0.72, all P 〈 0.05 ）. The expression levels of Notchl protein at post S status epileptieus 24 hours,day 3, day 7, day 15,1 month were 0.78 ＋_0.09,0.57 ±0.13,0.55 ±0.16,0.42 ±0.1

关 键 词：microRNA-34a 颞叶癫痫 NOTCHL 

分 类 号：R-332[医药卫生] R742.1