高效液相色谱法检测不同酒花浸膏中的9种味觉物质  

Determination of 9 Kinds of Taste Substances in Different Hops Extracts Using HPLC

作　　者：刘艺 

机构地区：[1]华宝香精股份有限公司,上海201821

出　　处：《食品工业》2017年第6期308-311,共4页The Food Industry

摘　　要：试验采用高效液相色谱法比较不同来源酒花浸膏对异合葎草酮(Humulone)、异葎草酮(Isohumulone)、异加葎草酮(Tetrahydroisoαacid)、合葎草酮(Cohumulone)、葎草酮(Humulone)、加葎草酮(Adhumulone)、合蛇麻酮(Colupulone)、蛇麻酮(Lupulone)及加蛇麻酮(Adlupulone)9种味觉物质含量的影响。试验采用Waters YMCTMCarotenoid 3μm色谱柱(4.6 mm×150 mm),流动相A相为0.1%H_3PO_4(含0.2 mmol/L EDTA);流动相B相为乙腈,梯度洗脱,流速为1.0 m L/min,DAD检测波长为315 nm;柱温为40℃。检测方法显示:9种味觉物质的检测线性范围为0.01~500 mg/L;方法的检出限在0.02~0.2 mg/kg之间,在10~500μg/g的加标条件下的加标回收率在97.48%~105.12%之间,精密度为1.25%~3.02%(n=6),检测方法灵敏、有效。检测结果表明,不同来源酒花浸膏得到的酒花浸膏中异合葎草酮、异葎草酮、异加葎草酮、合葎草酮、葎草酮、加葎草酮、合蛇麻酮、蛇麻酮及加蛇麻酮9种酒花浸膏味觉物质的总含量以青岛大花酒花最高。In this study, the influence of different hops extracts on contents of nine kinds of the taste substances （humulone, isohumulone, tetrahydroiso a acid, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone） in hops extracts was compared by using HPLC. The experiment was based on Waters YMCTM Carotenoid 3 μm column （4.6 mm×150 mm） with mobile phase A of 0.1% H3PO4 （containing 0.2 mmol/L EDTA） and mobile phase B of acetonitrile, and gradient eluted with the flow rate of 1.0 mE/rain, DAD detection wavelength of 315 nm and column temperature of 40 ℃. Detection method showed that nine kinds of taste substances linear range were 0.1-500 rag/L; The method detection limit was between 0.02-0.2 mg/kg, precision of 1.25%-3.02% （n=6）, the recoveries were between 97.48%-105.12% in the spiked 10- 500 gg/g concentration. It was shown by the results that the total content of nine taste substances （humulone, isohumulone, tetrahydroiso a acid, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone） in extracts and the highest in Qingdao hops extracts.

关 键 词：酒花浸膏 异合葎草酮 味觉物质 高效液相色谱 

分 类 号：O657.72[理学—分析化学] TS262.5[理学—化学]