利用RNA-seq技术筛选大白猪皮下和肌内脂肪组织差异表达基因  

Identification of differentially expressed genes between subcutaneous and intramuscular adipose tissue of Large White pig using RNA-seq

作　　者：黄万龙[1] 张秀秀[1] 李嫒[1] 苗向阳[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《遗传》2017年第6期501-511,共11页Hereditas（Beijing)

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(编号:2013ywf-yb-5;2013ywf-zd-2);中国农业科学院农业科技创新项目(编号:ASTIP-IAS05);中国农业科学院创新基金项目(2004-院-1);国家高技术研究发展计划项目("863"计划)(编号:2008AA10Z140);国家重点基础研究发展计划项目("973"计划)(编号:2015CB943100)资助~~

摘　　要：为了比较分析大白猪皮下和肌内脂肪组织的全转录组数据,探究调控脂肪沉积的分子机制,本文采用RNA-seq技术和生物信息学方法鉴定大白猪皮下和肌内脂肪组织基因表达谱,对差异表达基因进行GO(Gene Ontology)分析、信号通路富集分析以及蛋白互作网络分析。大白猪皮下和肌内脂肪组织中有180个基因差异表达,上调基因主要参与细胞增殖、脂质激酶活性和磷脂代谢等与脂质代谢相关的生物学过程正调控,下调基因显著富集于脂肪细胞分化中起重要调控作用的MAPK信号转导通路。差异表达基因主要通过参与脂质代谢及通过MAPK信号转导通路调控脂肪细胞成脂分化,进而影响大白猪皮下和肌内脂肪的沉积。To explore the molecular mechanisms of lipid accumulation in different types of adipose tissue, the transcriptomes of the subcutaneous and intramuscular adipose tissues from the Large White pig were determined using RNA-seq technology and bioinformatics methods. The differential gene expression profiles were identified and analyzed with the Gene Ontology, KEGG pathway and protein-protein interaction network strategies. There were 180 differentially expressed genes between the two adipose tissues. The genes up-regulated in the subcutaneous,as compared to intramuscular, adipose tissues were mainly involved in the biological processes related to lipid metabolism. The down-regulated genes were significantly enriched in MAPK signaling pathway, suggesting that this signaling pathway could have an important regulatory role（s） in adipocyte differentiation. In summary, differentially expressed genes between the subcutaneous and intramuscular adipose tissues were predominantly involved in lipid metabolism/accumulation and regulation of adipogenesis in the Large White pig.

关 键 词：肌内脂肪 皮下脂肪 脂质代谢 MAPK信号通路 猪 RNA-SEQ 

分 类 号：S828[农业科学—畜牧学]