食管鳞癌细胞系TE7放射照射后miRNAs及DNA修复相关因子的表达  

Expressions of miRNAs and DNA damage repair factors in esophageal squamous cell carcinoma cell line TE7 after X-ray radiation

作　　者：赵晓静[1] 马军[1] 袁翎[2] 王昆仑[2] 李南[3] 张中冕[3] 孙佳[3] 

机构地区：[1]郑州大学第二附属医院消化内科,郑州450014 [2]郑州大学附属肿瘤医院河南省肿瘤医院放疗科,郑州450014 [3]郑州大学第二附属医院肿瘤科,郑州450014

出　　处：《郑州大学学报（医学版）》2017年第3期247-250,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技厅科技攻关项目152300410164

摘　　要：目的:探讨食管鳞癌细胞系TE7放射照射后miRNAs及DNA修复相关因子表达的关系。方法:采用实时定量PCR检测不同剂量(0、2、4、6、8 Gy)X射线照射后TE7中miR-126、miR-21、miR-101、DNA-PKcs mRNA及RAD21 mRNA的表达,蛋白免疫印迹法检测DNA-PKcs和RAD21蛋白的表达。结果:X射线照射后,随吸收剂量的增加,TE7中miR-126表达逐渐升高(P<0.05),miR-101表达降低(P<0.05),DNA-PKcs和RAD21 mRNA表达逐渐升高(P<0.05);磷酸化DNA-PKcs、总DNA-PKcs及RAD21蛋白表达无明显变化(P>0.05)。当吸收剂量是4Gy时,TE7细胞中miR-126、miR-101与RAD21蛋白表达呈负相关(r分别为-0.899、-0.853,P均<0.05)。结论:miR-126及miR-101可能通过调节RAD21的表达影响TE7细胞的放疗敏感性。Aim： To explore the expressions of miRNAs and DNA repair factors in esophageal squamous cell carcinoma cell line TE7 after radiation. Methods： Real-time PCR was used to detect the expressions of miR-126,miR-21,miR-101,DNA-PKcs,and RAD21 mRNA in TE7 cells treated with different doses（ 0,2,4,6,8 Gy） of X-ray radiation,and Western blot was used to detect the expressions of DNA-PKcs and RAD21 protein. Results： In TE7 cells treated with Xray radiation,the expressions of miR-126,DNA-PKcs and RAD21 mRNA were up-regulated with the dose increasing（ P 〈0. 05）,that of miR-101 was down-regulated（ P 〈 0. 05）,and the expressions of phospho-DNA-PKcs,total DNA-PKcs and RAD21 protein had no statistical changes（ P 〉 0. 05）. The expressions of miR-126 and miR-101 were negatively correlated with that of RAD21 protein（ r =-0. 899,-0. 853,P 〈 0. 05） in TE7 cells treated with 4 Gy radiation. Conclusion： MiR-126 and miR-101 may influence the radio-sensitivity of TE7 cells by regulating the expression of RAD21.

关 键 词：食管癌 MIR-126 MIR-21 miR-101 DNA-PKCS RAD21 放射照射 

分 类 号：R735.1[医药卫生—肿瘤]