结直肠癌患者组织中HIF-1α和GLS2表达水平与病理程度和预后的相关性  

Expression level of HIF-1α and GLS2 in the colorectal cancer tissue and its clinical significance

作　　者：葛丽卫 谢伟峰 顾金华[3] 黄支密 

机构地区：[1]浙江省湖州市解放军第九八医院检验科,313000 [2]浙江省湖州市解放军第九八医院普外科,313000 [3]浙江省湖州市中心医院检验科,313000

出　　处：《中华普外科手术学杂志（电子版）》2017年第3期265-268,共4页Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)

摘　　要：目的探讨结直肠癌组织中缺氧诱导因子-1α(HIF-1α)和2型谷氨酰胺酶(GLS2)表达水平与患者病理及预后的相关性。方法选取2015年3月至2016年3月行手术切除的结直肠癌组织89例,癌旁组织67例(距肿瘤5 cm以上),用免疫组织化学方法检测HIF-1α和GLS2的表达。应用SPSS 19.0统计学软件分析,HIF-1α和GLS2表达水平及与结直肠癌病理因素的关系采用卡方检验,患者预后的影响因素采用COX回归模型,以P<0.05表示差异有统计学意义。结果结直肠癌组织中HIF-1α的阳性率为64.0%(57例),GLS2的阳性率为67.4%(60例),明显高于癌旁组织(13.4%、19.4%),差异有统计学意义(P<0.05);结直肠癌组织中HIF-1α和GLS2的阳性率与肿瘤分期、分化程度和淋巴结转移有关;肿瘤分期为Ⅲ/Ⅳ期、分化程度差和淋巴结转移的结直肠癌组织中的HIF-1α和GLS2的阳性率显著高于肿瘤分期为Ⅰ/Ⅱ期、分化程度好和淋巴结未转移的结直肠癌组织(P<0.05);COX回归模型得出结直肠癌患者的HIF-1α、GLS2、肿瘤分期、分化程度和淋巴结转移均是患者预后的影响因素(P<0.05)。结论 HIF-1α和GLS2在结直肠癌组织中高表达、提示预后不良,与结直肠癌的发生、发展存在相关性,有望成为结直肠癌评估诊断的新靶点。Objective To discuss the correlation between the expression level of HIF-1α and GLS2 in the colorectal cancer tissue and the factor of pathology and prognosis. Methods The 89 cases of CRC tissues and 67 cases of para-carcinoma tissues （ distance the tumor more than 5 cm ） were collected from March 2015 to March 2016 in our hospital, the expression of IF-1 and GLS2S were detected by immunohistochemistry. SPSS 19.0 software was used to analyze the data. The relationship between the expression of HIF-1α and GLS2 and the pathology factor of colorectal cancer was examined by chi square test, the influence factor of the prognosis was examined by COX regression model, a P 〈 0. 05 was considered as significant difference. Results The positive rate of HIF-1α is 64.0% （57 cases） and GLS2 is 67.4% （60 cases） in the tumor tissue of colorectal cancer patients, compared with the para-carcinoma tissues, both significantly increased（ 13.4% , 19.4% ） ; The positive rate of HIF-1α and GLS2 in the tumor tissue of colorectal cancer patients have relation with tumor staging, differentiated degree and lymphatic node metastasis; The positive rate of HIF-1α and GLS2 of colorectal cancer patients with tumor stage is m or 1V stage, good differentiated degree and lymphatic node metastasis significantly higher than tumor stage is Ⅰ or Ⅱ stage, bad differentiated degree and without lymphatic node metastasis （ P 〈0. 05 ） ; COX regression model confirmed that the HIF-1α, GLS2, tumor staging, differentiated degree and lymphatic node metastasis of colorectal cancer patients is the influence factor of prognosis （P 〈 0.05 ）. Conclusion High expression of HIF-1α and GLS2 predicts poor prognosis in the patients with colorectal cancer, indicating that there is correlation with the occurrence and progression of the disease, serve as the new targets for assessment and diagnose of the colorectal cancer.

关 键 词：结直肠肿瘤 缺氧诱导因子1 Α亚基 谷氨酰胺酶 病理学 临床 

分 类 号：R735.34[医药卫生—肿瘤]