散发性大肠腺瘤性息肉患者粪便中APC基因突变的意义及与中医体质的关系  

Significance of APC gene mutation in stool of sporadic colorectal adenoma patients and its relationship with TCM body constitution

作　　者：刘杨[1] 巩阳[1] 林一帆[1] 杨卓[2] 冯玉霞[1] 

机构地区：[1]沈阳军区总医院中医科国家中西医结合消化病重点学科,辽宁沈阳110840 [2]沈阳军区总医院内镜中心,辽宁沈阳110840

出　　处：《中国中西医结合消化杂志》2017年第5期327-331,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：辽宁省科技发展攻关计划项目(No:2013225089)

摘　　要：[目的]观察粪便中散发性大肠腺瘤性息肉APC基因突变的意义,探讨易出现APC基因突变的中医体质类型。[方法]采用QIAamp DNA Stool Mini Kit 51504提取42例大肠腺瘤性息肉、8例非腺瘤性息肉、12例大肠癌患者及23例正常人群粪便的DNA,运用聚合酶链反应—单链构象多态分析法(PCR-SSCP)检测APC基因的突变情况;通过问卷形式调查散发性大肠息肉患者的中医体质类型。[结果]42例大肠腺瘤性息肉患者的粪便APC基因的突变率为21.43%(9/42);8例非腺瘤性息肉及23例正常组APC基因的突变率均为0;12例大肠癌患者粪便中APC基因突变率为33.33%(4/12);总突变率为15.30%。腺瘤性息肉组与正常组比较差异有统计学意义(P<0.05);腺瘤性息肉与大肠癌组比较差异无统计学意义(P>0.05)。粪便APC基因突变与中医体质无关(P>0.05)。[结论]检测粪便中APC基因的突变率有助于大肠腺瘤性息肉筛查,大肠腺瘤性息肉粪便中APC基因的突变与中医体质无明显关系。[Objective]To investigate the significance of APC gene mutation in sporadic colorectal polyps in feces,and to explore the TCM constitution type of APC gene mutation. [Methods]QIAamp DNA Stool Mini Kit 51504 was used for 42 cases of colorectal adenoma polyps,8 cases of none adenoma polyp,12 cases of patients with colorectal carcinoma and 23 cases of normal people fecal DNA extraction,polymerase chain reaction single stranded conformation polymorphism analysis was used to detect the APC gene mutation, and questionnaire survey was conducted among sporadic colonic polyps patients to estimate the TCM Con- stitution type. [Results]Fecal APC gene mutation rate was 21.43 % （9/42）in 42 cases of colorectal adenom- atous polyps patients;APC gene mutation rate was 0 in 8 patients with non adenomatous polyps and 23 cases of normal group; 12 cases of APC gene in stool of patients with colorectal cancer mutation rate was 33.33%（4/12） ;total mutation rate of 15.30%. There was significant difference between the normal group and the abnormal（colorectal adenoma） group（P〈0.05）. There was no significant difference in the contrast between the eolorectal adenoma group and the large bowel carcinoma（P〉0. 05）. The mutation of APC gene in feces was not correlated with traditional Chinese medicine body constitution（P〉0.05）. [Conclusion] Detection of the mutation rate of APC gene in feces is helpful to the screening of colorectal adenoma, and the mutation of APC gene in colorectal adenoma polyps has no significant relationship with TCM body constitution.

关 键 词：大肠腺瘤性息肉 粪便 APC基因 中医体质 

分 类 号：R574[医药卫生—消化系统]