高效低成本纤维素酶诱导物的制备、筛选及应用  

Preparation, Screening and Application of Cellulase Inducer with High Efficiency and Low Cost

作　　者：冯骏[1,2] 姚达行 李晨[2] 张东远[2] 肖冬光[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国科学院天津工业生物技术研究所天津市工业生物系统与过程工程重点实验室,天津300308 [3]广州市增城区农业科学研究所,广州511370

出　　处：《基因组学与应用生物学》2017年第6期2561-2568,共8页Genomics and Applied Biology

基　　金：国家863计划项目(2014AA021906);国家自然科学基金资助项目(编号:21406259)共同资助

摘　　要：本研究利用玉米芯、甘蔗渣、脱木素木糖渣及粗纤维诱导里氏木霉产纤维素酶,对4种材料进行成份测定,然后以逐步添加的方式与微晶纤维素混合诱导里氏木霉产纤维素酶,和使用微晶纤维素诱导产酶对比,玉米芯含有的纤维素代替总纤维素的50%时,酶活力降低2个单位,蛋白减少0.8 g左右,其酶水解能力降低0.4%,对其产纤维素酶的水解能力没产生不利影响。甘蔗渣纤维素替代量可以达到30%,酶活力有1个单位的降低,蛋白分泌降低0.5 g左右,酶的水解能力提高7%左右。脱木素木糖渣纤维素替代量也可达到50%,酶活力和蛋白降低分别达到0.5个单位和0.2 g左右,酶水解能力降低了4.45%。粗纤维的利用可以达到100%替代,对里氏木霉产酶的酶活力影响有0.3个单位之差,水解能力降低1.625%。这说明这几种物质可以部分替代或者完全替代微晶纤维素,诱导里氏木霉发酵产纤维素酶,特别是由玉米芯和甘蔗渣制备的脱木素木糖渣和粗纤维有着较高的应用前景。该研究对降低纤维素酶的生产成本及其工业化应用具有重要意义。In this study, the corncob, Sugarcane bagasse, delignification of xylose residue and crude fiber were used to induce T. reesei cellulase production. The four lignocellulosic material compositions were first analyzed, and then were mixed up with microcrystalline cellulose by stepwise addition method to induce T. reesei cellulase. When cellulose of corncob replaced 50% of the total cellulose, two activity unit of enzyme, 0.8 gram protein were reduced and the hydrolysis ability of its enzyme decreased by 0.4%. The hydrolysis ability of enzyme which produced cellulose was not influenced. When cellulose of sugarcane bagasse replaced 30% of the total cellulose, one activity unit of enzyme and 0.5 gram protein were reduced, but hydrolysis of cellulase increased by 7%. When the cellulose of delignification of xylose replaced 50% of the total cellulose, 0.5 activity unit of enzyme and 0.2 gram protein were reduced, and the cellulose hydrolysis ability reduced by 4.45%. When total cellulose was replaced by the cellulose of crude fiber, only 0.3 enzyme activities were changed, and the cellulose hydrolysisability reduced by 1.625%. It showed that these substances could partially or completely replace microcrystalline cellulose to induce cellulase production by the fermentation of T. reesei, especially the delignification of xylose residue and crude fiber made from corncob and bagasse. The study was meaningful for cutting the cost of cellulase production and its industrialization.

关 键 词：里氏木霉 诱导 纤维素 酶活力 

分 类 号：TQ925[轻工技术与工程—发酵工程]