高效液相色谱法同时分离检测食品添加剂  

Simultaneous separation and determination of food additives by high performance liquid chromatography

作　　者：校瑞 张晓婷[1] 牛家华[1] 秦培鸽 杨艺欣[1] 卢明华[1] 

机构地区：[1]河南大学化学化工学院,河南开封475004

出　　处：《化学研究》2017年第3期348-352,共5页Chemical Research

基　　金：国家自然科学基金(21477033);河南省高等学校青年骨干教师资助计划项目(2014GGJS-024);河南省高校科技创新人才支持计划(17HASTIT003)

摘　　要：建立了一种同时分离检测没食子酸丙酯(PG)、特丁基对苯二酚(TBHQ)、二丁基羟基甲苯(BHT)和苯甲酸等食品添加剂的高效液相色谱(HPLC)分析方法.通过对流动相、流速、柱温、检测波长等色谱条件的优化,确定了最佳的分离检测条件:甲醇(B)和水(A)(0.1%的乙酸溶液)作为流动相,梯度洗脱:0~6 min,60%B,流速0.3 m L/min;6~9 min,60%~100%B,流速0.6 m L/min,柱温维持在25℃,检测波长280 nm(PG、TBHQ、BHT),230 nm(苯甲酸).在最佳的色谱条件下,方法的线性范围分别为1~200、2~400、2~400、0.5~400 mg/L,检出限(LOD)为0.02~0.06 mg/L,相关系数(R2)均大于0.999 4,加标回收率在77.76%~106.27%之间.该方法还成功应用于话梅、薯片、方便面实际样品中食品添加剂的分析检测.A HPLC method for the analysis of food additives including propyl gallate(PG),tert-Butylhydroquinone(TBHQ),butylated hydroxytoluene(BHT) and benzoic acid in foods was developed.The chromatographic conditions with mobile phase,flow rate,column temperature were optimized,and methanol(B) and water with 0.1% acetic acid(A) were used as the mobile phase with that the gradient program is 0-6 min,60% B,the flow rate of 0.3 m L/min; 6-9 min,60%-100% B,the flow rate of 0.6 m L/min. The column temperature was maintained at 25 ℃. Under the optimized conditions,the linearity range of the method for the analysis of PG,benzoic acid,TBHQ,BHT were 1-200,2-400,2-400 and 0.5-400 mg/L with correlation coefficients(R2) with more than 0.999 4. The limits of detection(LODs) were in the range of 0.02-0.06 mg/L at a signal-to-noise ratio of 3,the recoveries of these compounds were in the range of 77.76%-106.27%. The developed method was also applied to analysis real samples.

关 键 词：高效液相色谱 同时分离检测 食品添加剂 抗氧化剂 防腐剂 

分 类 号：O65[理学—分析化学]