富硒香菇发酵液中硒多糖提取工艺的响应面优化  

Optimization of Extraction Process of Response From Selenium-polysaccharide Enriched Lentinus edodes in Fermentation Liquid Surface

作　　者：乔伟[1] 陈文强[1,2] 彭浩[1,2] 解修超[1,2] 邓百万[1,2] 乔艳明[1] 

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]陕西省食药用菌工程技术研究中心,陕西汉中723001

出　　处：《北方园艺》2017年第12期157-162,共6页Northern Horticulture

基　　金：陕西省"13115"科技创新工程计划资助项目(2008ZDGC-04)

摘　　要：以香菇9608#菌株为试材,在单因素试验基础上,采用Box-Behnken响应面设计法优化香菇发酵液中硒多糖提取工艺。结果表明:富硒香菇发酵液中提取工艺硒多糖最佳醇沉时间为15.45h、乙醇浓度82.30%、pH 7.95。在此条件下,富硒香菇发酵液中硒多糖产量达到3.23g·L^(-1),香菇发酵液中硒多糖硒含量87.67μg·g^(-1)。利用Box-Behnken响应面设计法得到的富硒香菇发酵液中硒多糖提取工艺参数,可为工业化生产提供必要的技术支持。Based on the single factor experiment,the method of Box-Behnken response surface design was used to optimize the extraction process of selenium-polysaccharide in Lentinus edodes fermentation broth. The results showed that the optimum alcohol extraction time of selenium-polysaccharide was 15. 45 hours, ethanol concentration 82.30% and pH 7. 95. Under these conditions, the yield of selenium-polysaccharide in the fermentation broth of selenium-enriched Lentinus edodes was 3. 23 g L-1 ,and the selenium content of selenium- polysaccharide was 87. 67 μg g-1. Using the Box-Behnken response surface design method, the extraction parameters of selenium-polysaccharide from the selenium-enriched Lentinus edodes fermentation broth could provide the necessary technical support for the industrial production.

关 键 词：香菇 硒多糖 BOX-BEHNKEN设计 回归模型分析 响应面分析 

分 类 号：S646.12[农业科学—蔬菜学]