四种芽孢杆菌对蒜薹灰葡萄孢霉抑制作用的时效性研究  

Timeliness of Four Bacillus Strains Against Botrytis cinerea of Garlic Sprouts

作　　者：魏倩[1] 张娜[2] 张平[2] 李明刚[1] 阎瑞香[2] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]国家农产品保鲜工程技术研究中心,天津市农产品采后生理与贮藏保鲜重点实验室,天津300384

出　　处：《生物技术通报》2017年第6期112-120,共9页Biotechnology Bulletin

基　　金：国家科技支撑项目(2015BAD16B07);河南省科技开发合作计划项目(152106000060).

摘　　要：旨在对保存的4种芽孢杆菌菌株种属水平做初步鉴定并研究其在离体条件下对蒜薹灰葡萄孢霉的抑制作用以及活体条件下对蒜薹保鲜的时效性。采用平板对峙法和菌丝生长法进行离体实验,采用有伤接种法在不同时间段进行预防和治疗活体实验。结果表明,离体条件下,与对照相比,4种芽孢杆菌对灰葡萄孢霉菌落扩展和菌丝体生长都有显著的抑制作用且效果HB-2>B579>B1>B001(P<0.05)。20℃活体实验中,4种芽孢杆菌的预防处理均能较好地控制蒜薹灰葡萄孢霉病斑扩展,且效果好于治疗处理,但是不同芽孢杆菌最佳的预防时间有所不同。其中,HB-2和B579预防处理能较好地保持蒜薹的可溶性固形物、维生素C以及可滴定酸含量。综合来说,芽孢杆菌HB-2比其他3种芽孢杆菌能更有效地预防蒜薹灰霉病。The aim of this work is to identify the four strains of Bacillus at the species level and study their inhibitory effects on Botrytis cinerea in vitro and their timeliness of fresh-keeping garlic sprouts in vivo. The plate-to-plate method and the mycelial growth method were usedfor in vitro experiments,and the injury inoculation method was for prevention and therapy treatment in vivo experiments in different periods.Results showed that four Bacillus strains in vitro presented significant inhibitory effects on colonization and mycelial growth of Botrytis cinereain contrast to the control,and in the order of HB-2 B579 B1 B001（P 0.05）. In vivo experiments under 20℃ showed that preventivetreatments with four Bacillus strains effectively controlled the expansion of B. cinerea lesion,and the effect was better than that of therapytreatment,but the optimal preventive time of various Bacillus varied. Among them,the preventive treatment with Bacillus HB-2 and B579 better maintained the soluble solids,vitamin C and titratable acid content of garlic sprouts. In conclusion,Bacillus HB-2 is more effective thanthe other three strains of Bacillus to prevent garlic mold of garlic sprouts.

关 键 词：芽孢杆菌 灰葡萄孢霉 蒜薹保鲜 时效性 

分 类 号：S436.33[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]