食品级纳米二氧化硅对小鼠器官功能影响的研究  

Study on the effects of the food grade silica Nanoparticles on the organ function of the mice

作　　者：刘嘉褀 候晓敏 彭子荷 刘和亮[3,4] 郝小惠[3,4] 

机构地区：[1]华北理工大学基础医学院,河北唐山063000 [2]华北理工大学公共卫生学院,河北唐山063000 [3]华北理工大学医学实验研究中心,河北唐山063000 [4]国家科技部老年医学国际科技合作基地,河北唐山063000

出　　处：《食品工业科技》2017年第13期292-296,共5页Science and Technology of Food Industry

基　　金：河北省科学技术研究与发展计划项目(10276116D);唐山市老年医学科技创新团队(15130212C)

摘　　要：目的:通过测定小鼠连续性口服食品级纳米二氧化硅(Nano silicon dioxide,Nano-SiO_2)12周后血清及肝、肾、睾丸组织中硅元素的含量,并对小鼠肝肾等器官进行观察,初步评价纳米二氧化硅对机体的安全性。方法:将雄性昆明小鼠随机分为对照组(0.05%BSA)、纳米SiO_2低剂量组(100 mg/kg/day)、中剂量组(500 mg/kg/day)、高剂量组(1000 mg/kg/day)和微米SiO_2组(1000 mg/kg/day),各组按上述剂量分别连续灌胃二氧化硅84 d后全部处死,利用HE染色观察各组动物的肝脏、肾脏、睾丸等组织病理学变化;ICP-OES检测血清及肝、肾、睾丸组织中硅元素的含量;检测各组动物血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)和尿素氮(BUN)的含量。结果:病理学观察在纳米低、中、高剂量组均发现肝脏、肾脏和睾丸组织有形态上的异常改变,以高剂量组更严重;纳米组血清中ALT、AST和BUN的含量均显著增高(p<0.01或p<0.05);同时在血清及肝、肾、睾丸组织中纳米组的硅离子含量增高并呈现剂量依赖性;上述指标中同等剂量纳米组和微米组相比,纳米组含量高且病理变化更明显。结论:较长时间口服摄入食品级纳米二氧化硅可能在机体的肝脏、肾脏、睾丸等脏器内蓄积,造成一定程度的肝脏、肾脏等的病理学损害并影响其功能。长期多量食入纳米二氧化硅可能会对机体造成不良影响。Objective：To evaluate the safety of Nano silica on the tissues of the mice,the mice were taken oral administration with the food grade silica Nanoparticles （Nano silicon dioxide, Nano-SiO2 ）, after 12 weeks the content of silica in serum, livers,kidneys and testis of silica were detected,and the livers and kidneys of mice were observed.Methods：Male KM mice were randomly divided into different groups including the control group （0.05 % BSA）, Nano- SiO2 groups （ 100,500,1000 mg/kg/day ） and micron-SiO2 group （1000 mg/kg/day）. 84 days later, all animals were killed and the tissues were collected. The histopathological changes of livers,kidneys,testis and other tissues of the mice by HE staining were obversed.Content of silicon in livers, kidneys, testis and serum were detected by ICP- OES. The content of alanine aminotransferase （ALT） , aspartate aminotransferase（AST） and blood urea nitrogen（BUN） in serum were measured by colorimetric method.Results ：All the results were compared with the control group. The HE staining showed the varying differences of pathological changes in the livers, kidneys and testieular tissue abnormalities and the high dose of Nano-SiO2 group was the most.With the increase of Nano-SiO2 doses,the contents of ALT, AST and BUN were increased.The examination of the silicon in the livers,kidneys,testis and serum of the Nano groups were inhibited in dose-dependent manner.Compared with the same dose of micron group,the above results of the Nano group were more serious. Conclusion： Taking orally with food grade Nanosilicon for a long time, the poison may accumulate in the body- liver, kidney, testis and other tissues,leading to different histopathological damages and affect their functions.

关 键 词：食品添加剂 纳米二氧化硅 ICP-OES 谷草转氨酶 谷丙转氨酶 

分 类 号：TS202.3[轻工技术与工程—食品科学]