梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管通透性的保护作用  

Study on the effect of Zige lyophilized powder for injection against the microvascular permeability after cerebral ischemia-reperfusion injury of rats in the celebral microcirculation

作　　者：尚远宏[1] 田金凤[1] 

机构地区：[1]攀枝花学院,四川攀枝花617000

出　　处：《食品工业科技》2017年第13期307-310,共4页Science and Technology of Food Industry

基　　金：四川省教育厅重点基金项目(14ZA0345;15ZA0370);攀枝花市社会发展科技计划项目(2016 CY-S-10);攀枝花学院博士科研启动经费项目(bkqj2015026;bkqj2015023);四川省高校重点实验室开放基金项目(GR-2015-E-01;szjj2017-110)

摘　　要：本文探讨梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管通透性的影响。选择雄性SD大鼠272只,随机分为假手术组、模型组、葛根素注射液组和梓葛冻干粉针3个剂量组。线栓法复制大鼠缺血再灌注模型,分别缺血2 h,于再灌注1、4、7、14 d给药后,取脑提取微血管,用RT-PCR法测定脑微血管中闭合蛋白(Occludin)、紧密连接蛋白-5(Claudin-5)、闭合小带蛋白-1(ZO-1)、基质金属蛋白酶-9(MMP-9)的mRNA表达。结果表明,与模型组相比,再灌注4、7、14 d,梓葛冻干粉针16.40、32.70、65.40 mg·kg^(-1)能抑制脑缺血/再灌注大鼠缺血侧脑皮质层微血管中MMP-9mRNA表达的升高(p<0.01);并促进微血管中Claudin-5、Occludin、ZO-1 mRNA表达(p<0.05)。说明梓葛冻干粉针通过抑制MMP-9基因表达,并促进Claudin-5、Occludin、ZO-1基因表达,进而保护和改善脑微血管通透性。This paper investigated the protective effect of Zige lyophilized powder for injection against the microvascular permeability after cerebral ischemia-reperfusion injury of rats in the celebral microcirculation.272 male sprague-dawley rats were randomly divided into sham group, model group ,puerarin injection group ,three groups of Zige lyophilized powder.It made a cerebral ischemia-reperfusion model using the line in the left middle cerebral artery occlusion （ MCAO ） of rats in ischemia for 2 h and reperfusion for 1,4,7 and 14 d, the model of rats was established by intravenous injection of drugs.The expression of MMP-9 ,ZO-1,Occludin and Claudin-5 mRNA of the brain microvessel in the lateral ischemic cerebral cortex was detected by fluorescence quantitative PCR.In reperfusion for 4,7 and 14 d, compared with model group ,Zige lyophilized powder groups （16.40,32.70 and 65.40 mg-kg-1） could reduce romp-9 mRNA level in microvessel of the lateral ischemic cerebral cortex （p 〈 0.01 ） ,and could increase claudin- 5, occludin, zo- 1 mRNA level （p 〈 0.05 ） in the corresponding time. These results suggested that Zige lyophilized powder for injection could improve and protect the microvascular permeability by reducing MMP-9 and increasing claudin-5 ,occludin,zo-1 mRNA level.

关 键 词：梓葛冻干粉针 CLAUDIN-5 OCCLUDIN ZO-1 MMP-9 脑微血管通透性 

分 类 号：TS201.4[轻工技术与工程—食品科学]