密蒙花黄色素提取工艺优化  

Process Optimization on Extracting Uranidin from Buddleja officinalis

作　　者：曹昌伟[1] 黄慧福[2] 王瑞[2] 杨丽芳[2] 杨兰兆 谷大海[1] 

机构地区：[1]云南农业大学,云南昆明650201 [2]曲靖师范学院,云南曲靖655011

出　　处：《贵州农业科学》2017年第6期119-122,共4页Guizhou Agricultural Sciences

基　　金：云南省科技厅省院省校合作专项项目"云南省特色资源风味调料产品开发及产业化"(2013IB010)

摘　　要：为密蒙花黄色素的综合开发利用提供参考,以云南密蒙花为试验材料,采用水相提取法,通过单因素试验考察提取时间、提取温度和料液比3因素对提取密蒙花黄色素吸光度值的影响,并利用响应曲面法优化其提取工艺。结果表明:通过响应曲面法建立密蒙花黄色素提取工艺的回归模型为y=2.51+0.059A+0.20B-0.14C+0.033AB+0.078AC+0.034BC-0.090A^2-0.14B^2-003C2,模型的决定系数R^2=0.913,该回归模型的拟合程度较好;最佳提取工艺为提取时间30min、提取温度90℃、料液比1∶150(g∶mL),此条件提取密蒙花黄色素液的吸光度值为2.624,与理论预测值2.635接近,应用响应曲面法优化得到的回归模型参数准确可靠。To provide references for integrated exploitation and utilization of uranidin from B. officinalis, aqueous phase extraction method was employed to extract uranidin from Yunnan B. officinalis, then a single factor experiment was conducted to explore the effects of extraction time, extraction temperature and ratio of solid to liquid on the absorbance of uranidin, and the extraction process was optimized by response surface method. Results： Regression model of extraction process of uranidin established by the response surface method is y=2.51＋0. 059A＋0.20B-0.14C＋0. 033AB＋0. 078AC＋ 0. 034BC- 0. 090A^2 - 0. 14B^2 ＋ 9. 15E- 003C^2, with determination coefficient of R^2 = 0. 913. The regression model is of fine fitting degree; The optimum conditions are as follows： extraction time 30 min, extraction temperature 90℃, ratio of solid to liquid 1： 150 （g ：mL）. Under these conditions the absorbance is 2. 624, close to the theoretical absorbance of 2. 635, the parameters of the regression model obtained through response surface method are accurate and reliable.

关 键 词：密蒙花 黄色素 水相提取 响应面法 优化工艺 

分 类 号：TS202[轻工技术与工程—食品科学]