紫薇‘香雪云’香气成分时空动态变化研究  

Study on spatial-temporal variability of volatile components in Lagerstroemia indica 'Xiang Xue Yun'

作　　者：徐婉[1] 蔡明[1] 潘会堂[1] 张启翔[1] 

机构地区：[1]花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室林木花卉遗传育种教育部重点实验室北京林业大学园林学院

出　　处：《北京林业大学学报》2017年第6期85-95,共11页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(31500574);北京市青年英才计划项目(YETP0743);福建省科技计划项目(2014N3012);北京市共建项目专项资助(2016GJ-03)

摘　　要：为了研究紫薇‘香雪云’香气成分的时空动态变化,本文利用顶空-固相微萃取结合气相色谱-质谱(HSSPME-GC-MS)联用技术,分析‘香雪云’花朵挥发性成分在4个开花时期(半开期、盛开期、盛开末期、衰败期)和盛开期花朵的3个花器官部位(花瓣、雄蕊、雌蕊)中的释放规律。结果表明:1)从紫薇‘香雪云’花朵的4个开花时期中,共检测出80种挥发性成分,分别属于11个类别;倍半萜类、单萜类和苯类化合物是其中具有较高质量分数的化合物类别;α-法尼烯、松香芹酮和苯乙醇等是盛开期中具有较高质量分数的挥发性成分。2)在紫薇‘香雪云’的不同开花阶段,各挥发性成分的释放规律不同;共呈现出6种主要的挥发模式,即持续下降模式、先下降后上升模式、先下降后上升再下降模式、持续上升模式、先上升后下降模式和先上升后下降再上升模式。3)就花器官而言,花瓣中的苯类化合物和萜类化合物的质量分数均较高;雌蕊和雄蕊中则均以萜类化合物为主,但化合物种类及其质量分数也存在较大差异。研究结果为了解紫薇属植物的香气成分奠定了基础,为紫薇香花定向育种提供了依据。This study aims to determine the spatial-temporal variability of volatile components of Lagerstroemia indica ‘Xiang Xue Yun ＇ by headspace solid micro-extraction（ HS-SPME） coupled with gas chromatography-mass spectrometry（ GC-MS）. The aroma compounds of flowers at four different developmental periods（ middle-opening period, full-opening period, late-blooming period, bloomdeclining period） and three floral organs（ petal,stamen,pistil） in full-opening period were studied. The results showed that： 1） eighty volatile compounds belonging to 11 categories were identified from flowers during the whole flowering process of L. indica ‘Xiang Xue Yun ＇, of which, sesquiterpenes,monoterpenes and benzenoids were the main categories with high mass fraction and α-farnesene,pinocarvone and phenylethyl alcohol were the major volatile components emitted with high mass fraction.2） Volatile compounds emitted from L. indica ‘Xiang Xue Yun ＇flower during flowering showed six different modes： the continuely-declining pattern; declining first and then rising pattern; the pattern of declining first,rising later and then declining again; continuely-rising pattern; rising first and then declining pattern; the pattern of rising first,later declining and then rising again. 3） In petals,the massfraction of benzenoids and terpene compounds was high,while in pistil and stamen,the relative content of terpene compounds was high. But the volatile compounds and their mass fraction were distinctly different at different floral organs of L. indica ‘Xiang Xue Yun＇. This study lays a good foundation to understand the volatile organic compounds of Lagerstroemia plants and to provide the basis for fragrant breeding of crape myrtle.

关 键 词：紫薇'香雪云’ 挥发性成分 顶空-固相微萃取 时空表达 

分 类 号：S685.99[农业科学—观赏园艺]