海洋细菌GM-1-1菌株摇瓶发酵培养基和发酵条件优化  

Optimization of fermentation medium components and cultural conditions for marine bacteria GM-1-1 in flask

作　　者：曹雪梅[1] 汪晶晶[1] 贾杰 李欢[1] 王军强[1] 王琦[1] 马桂珍[1] 暴增海[1] 

机构地区：[1]淮海工学院海洋学院,连云港222005 [2]江苏耕耘化学有限公司,连云港222005

出　　处：《食品科技》2017年第6期23-29,共7页Food Science and Technology

基　　金：江苏省科技厅(现代农业)重点研发计划项目(BE2016335);连云港市科技局产业前瞻与共性关键技术项目(CG1513);国家级大学生实践创新训练计划项目(G201411641105002);江苏省“十二五”高等学校水产类重点专业项目

摘　　要：GM-1-1菌株是从连云港海域分离到的一株对多种病原真菌具有较好抑制作用的海洋解淀粉芽孢杆菌(Bacillus amyloliquefaciens),为了提高该菌株发酵液的抑菌活性,以发酵液的菌体密度及对白色念珠菌(Candida albicain)的抑制作用为检测指标,采用单因素试验和正交试验对GM-1-1菌株的摇瓶发酵培养基成分及发酵条件进行优化。研究结果表明,GM-1-1菌株生长及产生抑菌物质的最佳培养基配方为:糊精20 g/L,牛肉膏15 g/L,MgSO_4 1.4 g/L,pH7.0;最佳发酵条件为:温度28℃,摇床转速220 r/min,培养时间28 h,种子液浓度10~8 cfu/mL时接种量为10%,装液量为50 mL(250 mL三角瓶)。The marine bacteria GM-1-1 strain in this experiment was isolated fromLianyungang sea. In order to investigate best conditions for liquid fermentation and increase the antagonistic substance productivity of Bacillus amyloliquefaciens GM-1-1 strain. Cell density and the inhibitory effect of sterile liquid fermentation on Candida albicain as detection index, the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions. The results showed that the appropriate culture medium for the growth and the production of antimicrobial substances of marine bacteria GM-1-1 strain were dextrin 20 g/L, beef paste 15 g/L, MgSO4 1.4 g/L. The best fermentation conditions were pH 7.0, temperature 28 ℃, table speed 220 r/min, incubation time 28 h, seed density 10~8 cell/mL, 10% inoculum volume, and medium volume 50 mL/250 mL.

关 键 词：解淀粉芽孢杆菌 培养基 发酵条件 抑菌作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]