适配体生物传感器在赭曲霉毒素检测中的应用  

The application of aptamer based biosensor on ochratoxin detection

作　　者：赵阳阳[1,2] 吕蕾[3] 刘仁杰[1] 金永东[2] 郭志军[3] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]中国科学院长春应用化学研究所电分析国家重点实验室,长春130002 [3]延边大学农学院,延吉133002

出　　处：《食品科技》2017年第6期280-284,共5页Food Science and Technology

基　　金：吉林省教育厅科学技术研究项目(吉教科合字[2016]第252号);吉林省科技厅青年科研基金项目(20160520047JH);国家自然科学基金项目(31360384;31460423)

摘　　要：适配体是通过指数富集系统进化技术(SELEX)体外筛选得到的一类能够特异性地结合小分子物质、蛋白,甚至整个细胞的寡聚核苷酸序列。适配体具有制备简便、易于修饰、亲和力强,稳定性好等特点,已作为识别分子广泛应用于构建生物传感器。从以荧光信号、比色信号、电化学信号作为检测信号等方面进行综述适配体在检测食品中常见的毒素——赭曲霉毒素方面的应用。最后,对生物传感器在食品真菌毒素检测的发展前景进行了展望。Aptamers are single-stranded oligonucleotides of DNA or RNA sequences that are produced by in vitro selection process called the systematic evolution of ligands by exponential enrichment（SELEX）. These oligonucleotides are considered promising recognition elements for biosensor applications because of their advantages such as easier modification and immobilization, better stability, and higher reproducibility. In this paper, the application of aptasensor based on fluorescence signal, colorimetric method, and electrochemical signal for ochratoxin detection were reviewed. Meanwhile, the development of prospects of the aptasensor for food mycotoxins were reviewed.

关 键 词：适配体 生物传感器 赭曲霉毒素 

分 类 号：TS207.5[轻工技术与工程—食品科学]