紫玉米苞叶花色苷与牛血清白蛋白相互作用研究  

Study on the Interaction between Purple Corn Bract Anthocyanins and Bovine Serum Albumin

作　　者：朱莉[1] 马越[1] 赵晓燕[1] 王丹[1] 

机构地区：[1]北京市农林科学院蔬菜研究中心农业部华北地区园艺作物生物学与种质创制重点实验室农业部都市农业(北方)重点实验室,北京100097

出　　处：《食品研究与开发》2017年第12期13-16,共4页Food Research and Development

基　　金：北京市农林科学院青年科研基金(QNJJ201603);北京市科技新星计划(Z131105000413023)

摘　　要：采用荧光光谱法、紫外可见吸收光谱法、高效液相色谱法研究紫玉米苞叶花色苷与牛血清白蛋白(BSA)之间的相互作用。结果表明,紫玉米苞叶花色苷与BSA之间存在一定的相互作用,结合程度随着紫玉米苞叶花色苷浓度的增加而增加,二者结合后,会引起BSA结构的变化。紫玉米苞叶中6种主要的花色苷成分与BSA结合程度不同,其结合能力大小依次为:天竺葵-3-(6'-丙二酰葡萄糖苷)>芍药-3-(6'-丙二酰葡萄糖苷)>天竺葵-3-葡萄糖苷>矢车菊-3-葡萄糖苷>矢车菊-3-(6'-丙二酰葡萄糖苷)>芍药-3-葡萄糖苷。Fluorescence spectrometry, UV-VIS absorption spectroscopy, and high-performance liquid chro- matography （HPLC） were used to research the interaction between purple corn bract anthocyanins and bovine serum albumin （BSA）. The results showed that there was a relationship on the purple corn anthocyanins bract and BSA. The bonding degree increased with the increase of the concentration of anthocyanins . Furthermore, the structure of BSA was changed after the combination. The binding abilities of six anthocyanins in purple corn bract was different. The order of combining ability was as follows- pelargonidin-3-（6j -malonylglucoside）〉pe- onidin-3-（6＇-malonylglucoside）〉pelargonidin-3-goucoside〉cyanidin-3-glucoside〉cyanidin-3-（6＇-malnyyl- glucoside）〉peonidin-3-glucoside.

关 键 词：紫玉米 花色苷 荧光光谱 紫外光谱 高效液相色谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]