有机干扰物对超氧化电位水杀菌效果影响研究  

Study on the Influence of Organic Interfering Substance on Germicidal Efficacy of Neutral Electrolyzed Oxidizing Water

作　　者：陈红运[1] 宋全厚[1,2] 尹建军[1,2] 侯玉柱[1,2] 刘秀[1,2] 

机构地区：[1]中国食品发酵工业研究院,北京100027 [2]国家食品质量监督检验中心,北京100027

出　　处：《食品研究与开发》2017年第12期188-191,共4页Food Research and Development

基　　金：国家重点研发计划生物安全重点专项(2016YFC1201504)

摘　　要：试验以牛血清白蛋白(BSA)为有机干扰物质,大肠杆菌为灭菌对象,通过悬液定量杀菌试验和理化分析方法研究有机干扰物质对超氧化电位水(NEOW)杀菌效果影响。试验结果显示:在不含有机干扰物时,NEOW作用30 s,能够将菌落数对数值为7.76的大肠杆菌完全杀灭。用无菌水对NEOW稀释后,pH和氧化还原电位(ORP)变化不明显,有效氯浓度(ACC)随着稀释度的增加而降低,但稀释至60%时同样保持快速杀灭能力。在干扰物作用下,随着BSA浓度增加,NEOW杀菌效果降低。但NEOW在低浓度BSA的干扰下,可以通过增加杀菌时间或者增加有效氯浓度达到杀菌效果;在高浓度BSA的干扰下,需要对消毒环境进行预先清洗降低干扰物的量再进行消毒。Bovine serum albumin （BSA） was as organic interfering substances, Escherichia coli was for steril- ization object in the laboratory. Suspension quantitative germicidal test and physicochemical analysis method were carried out to observe the influence of organic interfering substance on germicidal efficacy of neutral elec- trolyzed oxidizing water （NEOW）.The results showed that when without organic interfering substances, the killing logartithm of Escherichia coli exposed to the NEOW for 30 s was 7.76. pH and oxidation reduction po- tential （ORP） changesd were not obvious after dilution of NEOW. Available chlori-ne concentration （ACC） de- creased as dilution, however, the 60 % of dilution of NEOW maintained same germicidal efficacy. Germicidal efficacy of NEOW was significantly reduced as the BSA concentration increased. But germicidal efficacy of NE- OW could be available by increasing time or ACC at low conce-ntrations of BSA, it need to be cleaned to re- duce the amount of organic interfering substances and then disinfected at high concentrations of BSA.

关 键 词：有机干扰物 大肠杆菌 超氧化电位水 杀菌效果 

分 类 号：TS205[轻工技术与工程—食品科学]