HPLC-ELSD法检测葛根酶解物中纤维低聚糖的含量  

Determination of Cello-Oligosaccharide in Radix Puerariae Enzymatic Preparation by HPLC-ELSD

作　　者：王朋凯 张雁[2] 唐小俊[2] 池建伟[2] WANG Peng-kai ZHANG Yan TANG Xiao-jun CHI Jian-wei(College of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, Hubei, China Sericultural ＆ Agri-Food Research Institute of Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, Guangdong,China)

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]广东省农业科学院蚕业与农产品加工研究所/农业部功能食品重点实验室/广东省农产品加工重点实验室,广东广州510610

出　　处：《食品研究与开发》2017年第13期145-149,共5页Food Research and Development

基　　金：广东省工业高新技术项目(2013B010102011);广州市产学研协同创新重大专项(201508020036);广东省应用型科技研发专项(2015B020230005)

摘　　要：建立高效液相色谱-蒸发光检测器(High Performance Liquid Chromatography-Evaporative Light Scattering Detection)测定葛根酶解制备物中葡萄糖、纤维二糖和纤维三糖的方法。HPLC条件为:采用Asahipak NH2P-50-4E(4.6 mm×250 mm,5μm)色谱柱,柱温35℃、流动相:乙腈∶水=70∶30(体积比)、流速0.8 mL/min;ELSD参数为:漂移管温度70℃,N2流速2.0 L/min。结果表明,葡萄糖、纤维二糖和纤维三糖的浓度与其峰面积在0.07 mg/mL^0.7 mg/mL内有良好的线性关系(R2>0.99),精密度RSD 0.99%~3.1%,平均回收率98.17%~103.42%。A high performance liquid chromatography （HPLC） with evaporative light scattering detection （ELSD） method was developed for the dermination of 3 saccharides such as glucose,cellobiose and cellotriose in the enzymatic hydrolysate of Radix Puerariae. A Asahipak NH2P-50-4E（4.6 mm×250 mm,5μm）column was used and the HPLC contidions were as follows：colunmn temperature 35℃, mobile phase：acetonitrile：water=70：30, flow rate 0.8 mL/min . And also the ELSD conditions were：detector drift tube temparature 70℃, nitro-gen flow rate 2.0 L/min. The results showed that there were good linear correlations between the concentration and the peak area of the 3 kinds of saccharides with the detection limitation from 0.07 mg/mL to 0.7 mg/mL（R^2〉0.99）.Precision RSDs of standard samples for the determination of the sugars were 0.99%-3.1%and average recoveries for the sugars in 5 real samples were 98.17%-103.42%.

关 键 词：高效液相色谱 蒸发光检测器 葛根纤维低聚糖 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]