黑鱼鱼胆抗炎活性成分研究  

Study on anti-inflammatory activities of bile from Channa argus

作　　者：吴小东[1] 戚微岩[1] 董瑶[1] 葛闯[1] 徐寒梅[1] 

机构地区：[1]中国药科大学多肽药物创制工程研究中心,江苏南京211198

出　　处：《中国药理学通报》2017年第7期941-945,共5页Chinese Pharmacological Bulletin

基　　金：江苏高校优势学科建设工程资助项目

摘　　要：目的研究黑鱼鱼胆的抗炎成分活性。方法采用萃取、硅胶柱层析等方法进行分离纯化,然后通过氢谱、碳谱对分离出的单体进行结构鉴定,利用小鼠脾淋巴细胞增殖实验和脂多糖(lipopolysaccharide,LPS)诱导小鼠巨噬细胞RAW264.7释放一氧化氮(nitrogen monoxide,NO)实验评价黑鱼鱼胆抗炎活性成分。结果利用活性追踪的方法分离得到化合物牛黄胆酸钠(C1)和化合物牛黄鹅去氧胆酸钠(C2),且两个化合物对刀豆蛋白A(Concanavalin A,Con A)诱导小鼠脾淋巴细胞增殖实验的细胞相对活力分别为65.9%±11.7%、60.5%±9.4%,与模型组相比差异均具有极显著性(P<0.01);对LPS诱导小鼠巨噬细胞RAW264.7释放NO的量分别为(16.4±1.9)、(15.5±1.7)μmol·L^(-1),与模型组相比差异均具有极显著性(P<0.01)。结论黑鱼鱼胆中牛黄胆酸钠和牛黄鹅去氧胆酸钠具有一定的抗炎活性且对正常脾淋巴细胞和巨噬细胞无毒性作用。Aim To study the anti-inflammatory activity of the Channa argus bile.Methods The bile was isolated and purified by extraction and silica gel column chromatography.Then the compounds were identified by hydrogen and carbon spectra.The spleen lymphocytes proliferation assay and Lipopolysaccharide（LPS）induced mouse macrophage RAW264.7 releasing Nitrogen Monoxide（NO） experiment were used to evaluate the anti-inflammatory activity.Results Compound（C1） of sodium taurocholate and compound（C2） of sodium taurochenodeoxycholate were isolated by activity tracing.The cell relative viabilities of the two compounds on Concanavalin A（Con A） induced spleen lymphocytes proliferation assay were 65.9% ± 11.7%and 60.5% ± 9.4%,which were significantly different from the result of model group（P〈0.01）,respectively.The NO production of LPS-induced RAW264.7 release of NO was（16.4 ± 1.9） μmol·L^-1and（15.5± 1.7） μmol·L^-1,which were significantly different from the result of model group（P〈0.01）.Conclusion Sodium taurocholate and sodium taurochenodeoxycholate from Channa argus perform the anti-inflammatory activities but have no cytotoxic effect on spleen lymphocytes and macrophage.

关 键 词：黑鱼 抗炎 牛黄胆酸钠 牛黄鹅去氧胆酸钠 淋巴细胞 巨噬细胞 

分 类 号：R-332[医药卫生] R282.74