2株南极来源放线菌所产的二酮哌嗪类化合物研究  

Investigation on the Diketopiperazines produced by two strains of Antarctic-derived actinomycetes

作　　者：田小杏 Kumar Saurav 张庆波[2] 张文军[2] 蒲晓辉[1] 张长生[2] 张海波[2] TIAN Xiao-xing KUMAR Saurav ZHANG Qing-bo ZHANG Wen-jun PU Xiao-hui ZHANG Chang-sheng ZHANG Hai-bo(Institute of Materia Medica, Henan University, Kaifeng 475004, China CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China)

机构地区：[1]河南大学药物研究所,河南开封475004 [2]中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室,中国科学院海洋微生物中心,广东省海洋药物重点实验室,广东广州510301

出　　处：《中国海洋药物》2017年第3期67-72,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(U1304826);广东省自然科学基金项目(2016A030313158);河南省科技发展计划项目(152102310077)资助

摘　　要：目的对从南极潮间带沉积物样品中分离得到的2株具有抗菌活性的放线菌进行分类鉴定并进行次生代谢产物研究。方法通过形态学分析及构建系统发育进化树鉴定菌株并对其发酵产物进行生物活性评价;利用硅胶、凝胶柱层析、semi-HPLC等色谱分离手段对2株菌的发酵产物进行分离纯化;采集所得化合物的质谱、NMR等数据并分析后鉴定其结构。结果 2株菌分别鉴定为Streptomyces sp.SCSIO 40061和Nocardiopsis sp.SCSIO KS107;从相应的发酵产物中分离得到2个二酮哌嗪类化合物,结构分别鉴定为:(3Z,6E)-1-N-甲基-3-苯亚甲基-6-(2-甲基-3-羟基丙烷)-2,5-二酮哌嗪(1)以及(3Z,6Z)-3-(4-对甲氧苯亚甲基)-6-(2-甲基丙烷)-2,5-二酮哌嗪(2)。结论发现了2株能产二酮哌嗪类化合物的南极来源放线菌。Objective To isolate and identify secondary metabolites produced by two strains of Antarcticderived actinomycetes which inhabit in the intertidal zone of Antarctic ocean.Methods Strains were identified on the basis of morphology and 16 SrDNA sequence analysis.Organic extracts of the two strains fermentation broth were evaluated for lethal activity against Brine Shrimp and antibacterial bioactivity.Several chromatography methods were adopted to isolate the crude extract for purified compounds.Structures of the obtained compounds were elucidated on the basis of spectral data analyses.Results Two Antarctic-derived actinomycete strains were identified as Streptomycessp.SCSIO 40061 and Nocardiopsis sp.SCSIO KS107.Their fermentation broth contained two diketopiperazine compounds.These two compounds were identified as（3Z,6E）-1-N-methyl-3-benzylidene-6-（2-methyl-3-hydroxypropylidene）-piperazine-2,5-dione（1）and（3Z,6Z）-3-（4-methoxybenzylidene）-6-（2-methylpropylidene）-piperazine-2,5-dione（2）,respectively.Conclusion This work provided two Antarctic-derived actinomycetes which could produce diketopiperazine compounds.

关 键 词：南极放线菌 STREPTOMYCES sp.SCSIO 40061 NOCARDIOPSIS sp.SCSIO KS107 二酮哌嗪 

分 类 号：R931[医药卫生—生药学]