IPTG添加时机对大肠杆菌产α-酮基丁酸的影响  

Effects of IPTG addition time on fermentation ofα-ketobutyric acid in Escherichia coli

作　　者：李娟[1] 齐俊生[1] 王婷[1] 毛倩[1] 韩超[1] 李燕军[1,2] LI Juan QI Junsheng WANG Ting MAO Qian HAN Chao LI Yanjun(College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin 300457, China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]代谢控制发酵技术国家地方联合工程实验室,天津300457

出　　处：《发酵科技通讯》2017年第2期77-82,共6页Bulletin of Fermentation Science and Technology

摘　　要：探究IPTG不同添加时间对大肠杆菌Escherichia coli THRD/pWSK29-ilvA发酵生产α-酮基丁酸菌体生长、产酸、代谢副产物和耗糖的影响.摇瓶发酵结果表明:在菌体处于对数生长中后期,菌体OD600值为10~15时,添加IPTG进行诱导,菌体生物量相对较高为6.5g/L,α-酮基丁酸积累量最高达到16g/L,同时糖酸转化率较其他诱导时间高,且副产物乙酸含量明显降低.利用7.5L发酵罐对此诱导条件进行发酵验证,α-酮基丁酸质量浓度为22g/L,高于已有报道中8g/L的生产水平.The study investigated the effect of IPTG addition time on theα-ketobutyric acid producing strain Escherichia coli THRD/pWSK29-ilvA in the aspects of cell growth,productivity,byproducts formation and sugar consumption.The results indicated that when IPTG was supplemented after the OD600 reached 10to 15,the biomass reached as high as 6.5g/L with the titer ofα-ketobutyric acid 16g/L.The glucose/acid conversion ratio was the highest compared to other groups with different IPTG addition time,and the acetic acid concentration decreased significantly.The optimized IPTG induction time was applied in a 7.5Lfermentor,and the results showed that the titer ofα-ketobutyric acid reached 22g/L,higher than previously reported results.

关 键 词：IPTG 诱导时机 α-酮基丁酸 产量 

分 类 号：TQ922[轻工技术与工程—发酵工程]