假交替单胞菌遗传操作系统的研究进展  

Advances in genetic manipulation on Pseudoalteromonas

作　　者：羊燕燕 裘娟萍[1] 余志良[1] YANG Yanyan QIU Juanping YU Zhiliang(College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China)

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《发酵科技通讯》2017年第2期100-106,共7页Bulletin of Fermentation Science and Technology

基　　金：国家自然科学基金资助项目(31670114);浙江省自然科学基金资助项目(LY16C010002);国家海洋经济创新发展区域示范项目(12PYY001SF08)

摘　　要：假交替单胞菌能产生多种胞外活性物质,赋予其重要的基础研究和应用研究价值.目前已有多株假交替单胞菌完成基因组测序.基因组序列分析揭示不同的假交替单胞菌具有产生不同活性代谢产物的能力.假交替单胞菌遗传操作体系欠成熟,这极大地限制了假交替单胞菌的研究与应用.主要对近十多年来假交替单胞菌遗传操作系统研究进展进行综述,包括感受态制备,穿梭载体、自杀敲除载体及筛选标记的成功应用,以期为假交替单胞菌遗传操作系统的发展提供参考.Pseudoalteromonas was ubiquitous in environments and can produce diverse bioactive compounds. So far, several Pseudoalteromonas isolates have been sequenced and the bioinformatics analysis has revealed their abilities to produce different bioactive compounds.However,the lack of genetic manipulation system of Pseudoalteromonas greatly restricted their applications and studies at molecular level. Here, we describe the advances in genetic manipulation on Pseudoalteromonas,including the preparation of competent cells,the successful application of shuttle vectors,suicide knockout vectors and screening markers,which provide the reference for the development of genetic manipulation system in Pseudoalteromonas.

关 键 词：假交替单胞菌 遗传操作 接合转移 

分 类 号：Q933[生物学—微生物学]