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机构地区:[1]菏泽学院化学化工系,山东菏泽274015 [2]菏泽学院生命科学系,山东菏泽274015
出 处:《食品工业科技》2017年第14期11-15,19,共6页Science and Technology of Food Industry
基 金:山东省自然科学基金(ZR2014BL020);山东省高校科技计划项目(J14LC55);菏泽学院海外智力为国服务行动计划
摘 要:用循环伏安法制备了聚色氨酸修饰玻碳电极。研究了己烯雌酚在聚色氨酸修饰电极上的电化学行为,建立了一种直接测定动物性食品中己烯雌酚含量的新方法。实验结果表明:在p H=5.0的磷酸盐缓冲溶液体系中,己烯雌酚在聚色氨酸修饰玻碳电极上于-0.2~0.8 V范围内出现一对可逆的氧化还原峰,与裸玻碳电极相比较,聚色氨酸修饰电极对己烯雌酚的电化学反应具有促进作用。己烯雌酚浓度在5.00×10^(-8)~1.00×10^(-6)mol/L范围内与其氧化峰电流成正比,检出限为2.00×10^(-9)mol/L,对己烯雌酚溶液平行测定6次的RSD为2.4%。该修饰电极具有良好的灵敏度、选择性和稳定性,可用于牛肉、鱼肉及奶粉中己烯雌酚含量的测定,回收率为96.0%~102.3%,结果满意,方法具有实用价值。A poly( tryptophan) modified glassy carbon electrode( PTRY/GCE) was prepared by cyclic voltammetry. The electrochemical behavior of diethylstilbestrol( DES) at the fabricated electrode was studied.A new method for the determination of DES in animal food was established.The experimental results showed that in p H5.0 phosphate buffer solution( PBS) system,DES appeared a pair of quasi redox peaks in the potential range of-0.2 to 0.8 V on the PTRY/GCE. The modified electrode showed a catalytic effect to the electrochemical reaction of DES.The oxidation peak current was proportional the concentratio of DES with a linear range of 5.00 × 10^-8 to 1.00 × 10^-6 mol/L and a limit of detection was 2.00 × 10^-9mol/L. The RSD for 6paralled determinations was 2.4%.The poly( tryptophan) modified glassy carbon electrode had good sensitivity,selectivity,and stability.The method could be applied to the determination of DES in beef,fish and milk powder with recoveries of 96.0% -102.3%.The results were satisfactory,and the method had practical value.
分 类 号:TS207.3[轻工技术与工程—食品科学]
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