基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异  

Analysis of the Differences in Temperature Adaptability Between Buffaloes and Yellow Cattle Based on Transcriptome Data of Skin Tissues

作　　者：齐昱[1] 邢燕平[1] 潘静[1] 凌宇[1] 曹宇[1] 孟凡华[1] 荆文魁 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010019 [2]中华人民共和国二连浩特出入境检验检疫局,二连浩特011100

出　　处：《中国畜牧兽医》2017年第7期1906-1914,共9页China Animal Husbandry & Veterinary Medicine

基　　金：内蒙古自然科学基金(2016MS0349);内蒙古高等学校科学研究项目(NJZY16067)

摘　　要：为了找到与水牛和黄牛温度适应性差异相关的信号通路,本研究对3头成年水牛和3头成年黄牛的皮肤组织进行了转录组测序和生物信息学分析。结果显示,测序后水牛和黄牛皮肤组织分别得到59 369 167和69 837 009条reads,与参考基因组的唯一比对率分别为70.76%和75.55%。水牛和黄牛皮肤组织共有1 611个差异表达基因,其中801个在水牛皮肤中上调,810个在水牛皮肤中下调。GO分析结果显示,注释到细胞组分、分子功能、生物学过程的差异表达基因分别为316、263和278个。KEGG分析结果显示,差异表达基因共富集在33条信号通路上,其中神经活性配体—受体相互作用、氧化磷酸化、胆碱能突触、5-羟色胺能神经突触、GABA能突触、钙信号通路等信号通路可能与水牛和黄牛在温度适应性上的差异相关。综上所述,突触与氧化磷酸化可能与水牛和黄牛之间的温度适应性差异有关,本研究为进一步了解水牛和黄牛对温度适应性差异的分子机制和分子育种奠定基础。In order to find out signaling pathways that related to the differences in temperature adaptability between buffaloes and Yellow cattle,RNA-sequencing and bioinformatics analysis were performed on the skin tissues of 3adult buffaloes and 3adult Yellow cattle.The results showed that 59 369 167 and 69 837 009 reads were obtained from buffaloes and Yellow cattle skin,respectively.The percentages of the uniquely mapped reads in buffaloes and Yellow cattle were 70.76% and 75.55%,respectively.There were 1 611 differentially expressed genes in buffaloes and Yellow cattle skin,801 of which were up-regulated in buffaloes skin and 810 were down-regulated in buffaloes skin.GO analysis showed that there were 316,263 and 278differentially expressed genes classified to the cellular components,molecular functions and biological processes,respectively.KEGG analysis showed that the differentially expressed genes were enriched in 33 signaling pathways,some of which such as neuroactive ligand-receptor interaction,oxidative phosphorylation,cholinergic synapse,serotonergic synapse,GABAergic synapse andcalcium signaling pathway might related to the differences in temperature adaptability between buffaloes and Yellow cattle.In summary,the synapse and oxidative phosphorylation might be related to the differences in temperature adaptability between buffalo and Yellow cattle.This study would lay a foundation for further understanding the molecular mechanism of temperature adaptation differences between buffaloes and Yellow cattle and molecular breeding.

关 键 词：转录组 信号通路 差异表达基因 皮肤 水牛 黄牛 

分 类 号：Q78[生物学—分子生物学]