成人和儿童急性白血病常见融合基因表达的比较  

Expression comparison of common fusion genes between adults and children with acute leukemia

作　　者：唐艳萍[1] 蔡政民[1] 唐亚梅[1] 苏程琳[1] 谭晓玉[1] 谢雨萱 利基林 

机构地区：[1]广西医科大学附属肿瘤医院实验研究部,南宁市530021

出　　处：《广西医学》2017年第7期952-954,共3页Guangxi Medical Journal

基　　金：广西科学研究与技术开发计划(桂科攻1298003-2-8)

摘　　要：目的比较成人和儿童急性白血病常见融合基因表达的差异。方法采用多重巢式PCR技术检测159例成人和120例儿童初治急性白血病患者的常见融合基因,比较成人及儿童各融合基因的阳性率。结果儿童急性淋巴细胞白血病(ALL)患者白血病融合基因总阳性率为51.11%(46/90),高于成人ALL患者的25.53%(24/94)(P<0.05);其中儿童TEL/AML1及TLS/ERG融合基因阳性率高于成人(P>0.05)。儿童急性髓系细胞白血病(AML)患者白血病融合基因总阳性率为53.33%(16/30),高于成人AML患者的26.15%(17/65)(P<0.05);其中儿童AML1/ETO融合基因阳性率高于成人(P>0.05)。结论成人及儿童急性白血病融合基因的表达存在一定差异,临床上应有针对性地进行相关融合基因的检测和判读,以提高诊疗质量。Objective To compare the expressions of common fusion genes between adults and children with acute leukemia.Methods The expressions of common fusion genes were detected in 279 patients with initial acute leukemia,including 159 adults and 120 children,by multiplex nested PCR. Then the positive rates of all fusion genes were compared between the adults and the children. Results The overall positive rate of leukemia fusion genes in the children with acute lymphoblastic leukemia（ALL） was higher than that in the adults with ALL[51. 11%（46/90） vs. 25. 53%（24/94）; P〈0. 05]. The positive rates of TEL/acute myeloid leukemia（AML） 1 and TLS/ERG fusion gene in the children were higher than those in the adults（P〉0. 05）. The overall positive rate of leukemia fusion genes in the children with AML was higher than that in the adults with AML[53. 33%（16/30） vs. 26. 15%（17/65）; P〈0. 05 ],and the positive rate of AML1/ETO fusion gene in the children was higher than that in the adults（P〈0. 05）. Conclusion There are some differences in the expressions of fusion genes between adults and children with acute leukemia,and the corresponding detection and interpretation of related fusion genes should be carried out to improve the quality of diagnosis and treatment.

关 键 词：急性白血病 融合基因 巢式 聚合酶链反应 

分 类 号：R733.71[医药卫生—肿瘤]