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作 者:卢忠英[1] 鲁道旺[1] 陈仕学[1] 姚元勇[1]
机构地区:[1]铜仁学院材料与化学工程学院,贵州铜仁554300
出 处:《河南农业科学》2017年第7期137-141,共5页Journal of Henan Agricultural Sciences
基 金:梵净山特色苗药资源保护与开发产学研基地项目(黔教合KY字[2014]233);贵州省科技计划项目(黔科合LH字[2016]7310号)
摘 要:以竹荪为原料,用微波辅助法提取其膳食纤维。在单因素试验结果的基础上,采用响应面法对提取工艺进行优化,以微波功率、微波提取温度、提取时间、液料比为自变量,利用Box-Behnken的中心组合设计原理进行响应面设计,优化提取工艺参数。结果表明:竹荪水溶性纤维(SDF)的最佳提取工艺条件为微波功率490 W、微波提取温度60℃、提取时间10 min、液料比20∶1,此条件下竹荪SDF得率可达12.26%,非水溶性纤维(IDF)得率为74.34%,该SDF得率与SDF得率的最大估计值比较,相对误差约为0.65%,且重复性也很好,因此,该优化提取工艺参数准确可靠。Dictyophora was used as raw material for extraction of dietary fiber with microwave-assisted method. Response surface methodology( RSM) was used to optimize the extraction process based on single factor tests. Box-Behnken central composite design principles were used for response surface design,with the power of microwave,liquid-to-solid ratio,microwave-assisted extraction time and temperature as four parameters. The optimum extraction conditions were as follows:microwave power of 490 W,extraction temperature of 60 ℃,microwave-assisted extraction time of 10 min,and liquid-to-solid ratio of 20 ∶ 1. Under the above optimum extraction conditions,the actual yield of SDF from Dictyophora was 12. 26%,and the yield of IDF was 74. 34%. The relative standard deviation between the theoretical and practical yields was 0. 65%,and the results demonstrated a good reproducibility,indicating the effectiveness and reliability of the RSM model.
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