不同溶藻菌对小球藻破碎及油脂提取效果的影响  

Effects of algicidal bacteria on cell disruption and oil extraction of microalgae Chlorella vulgaris

作　　者：邓春芳[1] 崔岩[1] 章莹颖[1] 成家杨[1] 

机构地区：[1]北京大学深圳研究生院环境与能源学院,广东深圳518055

出　　处：《中国油脂》2017年第7期106-110,共5页China Oils and Fats

基　　金：深圳市科创委基础研究项目(JCYJ20150626110855791);海洋公益性行业科研专项经费项目(201305022)

摘　　要：为探究溶藻菌对微藻细胞破碎及油脂提取效果的影响,以小球藻为研究对象,选取5株溶藻菌进行混合培养。结果表明:不同溶藻菌对小球藻细胞破碎及油脂提取效果各不相同。不同溶藻菌的溶藻率依次为:Aeromona hydrophila(73%)>Pseudomonas flurescens(65%)>Bowmanella denitrificans(56%)、Bacillus cirulans(56%)>Kordia sp.(43%)。利用正己烷对小球藻油脂进行提取,提油率依次为:Bowmanella denitrificans(58.4%)>Kordia sp.(52.7%)>Pseudomonas flurescens(24.1%)>Bacillus cirulans(22.5%)>Aeromona hydrophila(21.1%)>对照组(10.4%)。其中Bowmanella denitrificans菌株在24 h内就能达到高溶藻率,且提油率相比对照组提高4.6倍。In order to investigate the effects of algicidal bacteria on the cell disruption and oil extraction of microalgae Chlorella vulgaris, with microalgae Chlorella vulgaris as the study object, five strains of algicidal bacteria Kordia sp., Bowmanella denitrificans, Aeromona hydrophila, Pseudomonas flurescens, and Bacillus cirulans were mixed cultured. The results showed that the effects of different algicidal bacteria on the cell disruption and oil extraction of microalgae Chlorella vulgaris were different.The aglae-lysing rate of Aeromona hydrophila, Pseudomonas flurescens, Bowmanella denitrificans, Bacillus cirulans and Kordia sp. were 73%, 65%, 56%, 56% and 43%, respectively. The oil was extracted from Chlorella vulgaris by n-hexane.The oil extraction rate of Bowmanella denitrificans, Kordia sp., Pseudomonas flurescens, Bacillus cirulans, Aeromona hydrophila and control group were 58.4%, 52.7%, 24.1%, 22.5%, 21.1% and 10.4%, respectively. Bowmanella denitrificans was the most potential algicidal bacterium with high aglae-lysing rate which could be reached in 24 h and the oil extraction rate was 4.6 times higher than that of the control group.

关 键 词：溶藻菌 小球藻 细胞破碎 油脂提取 

分 类 号：TE667[石油与天然气工程—油气加工工程]