原花青素对铁负荷大鼠肝脏功能及TfR2、hepcidin基因表达的影响  

Effects of proanthocyanidins on the liver function and TfR2,hepcidin gene expression in iron overload rats

作　　者：张文芳[1] 云少君[1] 冯翠萍[1] 何兴帅 褚东阳 郭李萌 吴艳丽[1] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《中国农业大学学报》2017年第8期57-63,共7页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(31271826);山西农业大学引进人才博士科研启动费(2013YJ30);山西农业大学科技创新基金(20132-02)

摘　　要：为探讨原花青素对铁负荷致大鼠肝损伤的影响,通过监测肝功能、病理切片及转铁蛋白受体-2(TfR2)、铁调素(hepcidin)基因表达,将40只SD大鼠随机分为对照组、原花青素组、铁负荷组和试验组(铁负荷+原花青素)。ICP法辅以肝脏普鲁士蓝染色判别铁负荷大鼠模型是否建立成功;相关试剂盒检测谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性;HE染色观察肝组织形态学变化;Real-Time PCR检测肝组织中TfR2、hepcidin基因表达的变化。与对照组相比,铁负荷组Fe及ALT、AST水平显著升高(P<0.05),肝组织结构严重紊乱,hepcidin基因的表达量显著下降(P<0.05),TfR2基因的表达无明显差异;原花青素组Fe显著降低(P<0.05),ALT、AST水平无显著性变化,肝组织结构正常,TfR2与hepcidin基因的表达量显著下降(P<0.05)。与铁负荷组相比,试验组ALT、AST水平显著降低(P<0.05),肝组织紊乱程度减轻,Fe含量、TfR2及hepcidin基因的表达量均略降低,但差异无统计学意义。原花青素可以抑制铁的摄入量;在机体铁过载状态下,原花青素抑制铁吸收的能力可以在一定程度上改善肝功能。This study was to discuss the effect of proanthocyanidins on the liver function of iron overload rats according to test the iron content,level of liver alanine aminotransferase（ALT）and aspartate aminotransferase（AST）,the liver pathological changes,transferrin receptor-2（TfR2）and hepcidin gene expression.Forty healthy male SD rats were randomly divided into 4groups：Control group,proanthocyanidins group,iron overload group and test group.The rat liver were collected.ICP method was then used to measure the content of Fe supplemented with the identification of liver Prussian blue stain to check whether the iron-overloading rat model was set up successfully.The levels of ALT and AST were measured by related kits.HE staining was used to decide the liver pathological changes.TfR2 and hepcidin gene expressions were examined by real-time PCR.Results comparison showed that the content of Fe and the level of ALT and AST in the overload group increased significantly（P〈0.05）.The liver tissue structure was disorder severely.hepcidin gene expression decreased significantly（P〈0.05）and TfR2 gene expression did not change significantly（P〈0.05）.Compared with the control group,the content of Fe decreased significantly in the proanthocyanidins group（P〈0.05）while the activity of ALT and AST did not change significantly.No obvious lesion was observed in the liver tissue.The gene expression of TfR2 and hepcidin both decreased significantly（P〉0.05）.Compared with the ironoverload group,the level of ALT and AST in the test group decreased significantly（P〈0.05）.The liver tissue disorder was reduced.The content of Fe,TfR2 and hepcidin gene expression all decreased,but there were no significant differences.In conclusion,proanthocyanidins could restrain the intake of iron.In the condition of the body＇s iron overload,proanthocyanidins can improve liver function to a certain extent through inhibiting iron absorption.

关 键 词：铁负荷 原花青素 谷丙转氨酶 谷草转氨酶 HEPCIDIN TfR2 

分 类 号：TS201.4[轻工技术与工程—食品科学]