Halobacterium salinarium 5L发酵罐中产细菌视紫红质(BR)发酵条件优化  

Fermentation conditions optimization of producing bacteriorhodopsin by Halobacterium salinarium in a 5 L fermentor

作　　者：董菲菲[1] 张永杰[1] 穆航帆 张洪[1] 

机构地区：[1]河南大学生命科学学院,生物工程研究所,河南开封475004

出　　处：《食品工业科技》2017年第15期164-168,共5页Science and Technology of Food Industry

基　　金：河南省科技攻关项目(142102210392)

摘　　要：优化盐沼盐杆菌(Halobacterium salinarium)在5 L发酵罐中的生物量及细菌视紫红质含量的发酵条件,为该菌株的进一步应用提供依据。通过单因素实验考察温度、p H、搅拌转速、通气量和不同流速的玉米浆对盐沼盐杆菌的影响。优化后发酵条件为:温度37℃,转速250 r/min,通气量1.3 vvm,p H在发酵的过程中不用酸液和碱液调节,补加玉米浆的流速为0.2 m L/min。在此条件下,菌体的生物量(用OD_(600)表示)达到4.531,细菌视紫红质的含量达到90.86 mg/L,与未优化的条件相比分别提高了43.8%、36%。The technological conditions for bacteriorhodopsin and biomass by the Halobacterium salinarium in a 5 L fermenter were optimized,in order to provide the basis for further application of the strain.The effects of temperature,pH, agitation speed, ventilation volume and corn pulp with different flow rates on the Halobacterium salinarium were investigated by single factor experiments.The optimum fermentation conditions were temperature at 37 ＂C, agitation speed at 250 r/min, ventilation volume at 1.3 vvm,flow rate of corn pulp at 0.2 mL/min.pH was not regulated by acid and alkali in the fermentation process. Under these conditions,the maximum biomass was 4.531, the maximum BR yield was 90.86 mg/L,which was about respectively 43.8% ,36% greater than those obtained with initial fermentation conditions.

关 键 词：细菌视紫红质 培养条件 盐沼盐杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]