维生素D_3联合抗坏血酸对豚鼠结肠炎的改善作用  

EFFECT OF VITAMIN D_3 COMBINED WITH ASCORBIC ACID ON COLITIS IN GUINEA PIGS

作　　者：张晶[1] 孙睿森 马莹[1] 冯宏娟 李秀花[1] 邱服斌[1] ZHANG Jing SUN Rui-sen MA Ying FENG Hong-juan LI Xiu-hua QIU Fu-bin(School of Public Health, Shanxi Medical University, Taiyuan 030001, China)

机构地区：[1]山西医科大学公共卫生学院,太原030001

出　　处：《营养学报》2017年第3期265-269,共5页Acta Nutrimenta Sinica

基　　金：山西省基础研究计划项目(No.2014011044-1);国家自然科学基金项目(No.81573156)

摘　　要：目的探讨维生素D_3(VD_3)联合抗坏血酸(VC)对豚鼠溃疡性结肠炎的作用。方法将40只雄性豚鼠按体质量随机分为5组,每组8只:正常组[N,120mg VC+2.8μg VD_3/(kg·d)]、模型组[M,120mg VC+2.8μg VD_3/(kg·d)]、高VC低VD组[HCLD,240mg VC+1.4μg VD_3/(kg·d)]、中VC低VD组[MCLD,120mg VC+1.4μg VD_3/(kg·d)]和低VC低VD组[LCLD,8mg VC+1.4μg VD_3/(kg·d)]。每组均进食缺乏VD和VC的特殊饲料,采用改良的豚鼠灌胃方法给予各组相应剂量的VD_3和VC。灌胃7w后,N组继续饮用蒸馏水,其他4组饮用3%葡聚糖硫酸钠溶液3d,造模期间,灌胃情况同前。结果 N组体质量、粪便和精神状态正常,评分明显低于其他4组,血清中25-(OH)D_3、1,25-(OH)_2D_3显著升高,C反应蛋白(CRP)、白介素-6(IL-6)和核转录因子κB(NF-κB)明显降低;与LCLD组相比,HCLD组大体形态改善,组织学损伤较轻,疾病活动指数评分较低,血清中25-(OH)D_3、1,25-(OH)_2D_3显著升高,CRP、IL-6和NF-κB明显降低;M组评分显著低于LCLD组,其25-(OH)D_3、1,25-(OH)_2D_3显著高于LCLD组,CRP、IL-6和NF-κB较HCLD、MCLD和LCLD组明显降低,且均有统计学意义(P<0.05)。结论维生素D_3缺乏时,较高剂量的VC对豚鼠溃疡性结肠炎的防治效果更明显。Objective To investigate the effect of vitamin D3 combined with ascorbic acid on ulcerative colitis in guinea pigs. Methods Forty male Dunkin-Hartley guinea pigs were randomly divided into 5 groups according to their weight, with 8 guinea pigs in each group：normal group（N, 120 mg VC＋2.8μg VD3/（kg·d））, model group（M, 120 mg VC＋2.8μg VD3/（kg·d））, high VC and low VD group（HCLD, 240 mg VC＋1.4μg VD3/（kg·d））, middle VC low VD group（MCLD, 120 mg VC＋1.4μg VD3/（kg·d）） and low VC low VD group（LCLD, 8mg VC＋1.4μg VD3/（kg·d））. Guinea pigs in each group were fed a special diet without vitamin D and vitamin C, and the corresponding doses of VC and VD were given by gavage. After 7 weeks, the normal group continued drinking distilled water, and the other 4 groups were treated with 3% dextran sodium sulfate solution for 3 days. Within three days, the intervention was unchanged. Results N group had normal body weight, stool and mental status, and scores of N group were significantly lower than those in the other 4 groups. The serum 25-（OH）D3, 1,25-（OH）2D3 increased significantly. C-reactive protein（CRP）, interleukin-6（IL-6） and nuclear factor kappa B（NF-κB） were significantly decreased. Compared with LCLD group, gross morphology was improved in the HCLD group. Meanwhile, histological damage was less severe, disease activity index score was lower, serum 25-（OH）D3, 1,25-（OH）2D3significantly increased, and CRP, IL-6 and NF-kappa B significantly decreased. The score of M group was significantly lower than that of the LCLD group, while 25-（OH）D3, 1,25-（OH）2D3 was significantly higher than that of LCLD group, and CRP, IL-6 and NF-kappa B was significantly lower than that of HCLD, MCLD and LCLD groups（P〈0.05）. Conclusion The higher dose of VC was more effective in the prevention and treatment of ulcerative colitis in guinea pigs when vitamin D3 was deficient.

关 键 词：维生素D3 抗坏血酸 溃疡性结肠炎 豚鼠 CRP IL-6 NF-κB 

分 类 号：R574.62[医药卫生—消化系统]