云南肺癌易感性与HLA-A,HLA-B,DRB1,DQB1等位基因频率的相关性研究  

A Correlative Study on Lung Cancer with the Frequency of HLA-A, HLA-B,DRB1, DQB1 Alleles in Yunnan Province

作　　者：刘铮 印卫锋[2] 任晔[2] 郝萍[3] 王江[2] 

机构地区：[1]武汉市东西湖区人民医院肿瘤科,湖北武汉430040 [2]华中科技大学同济医学院附属同济医院骨科,湖北武汉430030 [3]昆明医科大学第一附属医院临床医学实验研究中心,云南昆明650032

出　　处：《现代生物医学进展》2017年第21期4038-4042,4062,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30260103);云南省自然科学基金项目(2002C0053M)

摘　　要：目的:测定云南肺癌患者人类白细胞抗原(human leukocyte antigen,HLA)-A、B、DRB1、DQB1等位基因出现频率,探讨HLA各等位基因位点与云南省肺癌发病易感性的相关性。方法:采用病例-对照相关分析方法,选取云南籍肺癌患者和健康个体各30例,应用序列特异性引物聚合酶链反应(polymerase chain reaction-sequence specific primer,PCR-SSP)对HLA-A、HLA-B、HLA-DRB1及HLA-DQB1等位基因频率进行测定,与正常组对比测算相对危险因子(relative risk,RR)。结果:肺癌组的HLA-A~*02频率为90.0%(A~*0201为主),B~*46频率为40.0%,DRB1~*15频率为40.0%,较对照组的43.30%、0%、10.0%明显升高(Pc<0.05,RR>1)。肺癌组的HLA-A~*31频率为3.30%,A~*33频率为6.70%,B~*27频率为3.30%,B~*52频率为6.70%,DRB1~*03频率为0%,DRB3~*01频率为60.0%,DQB1~*02频率为0%,DQB1~*06频率为0%,较对照组的23.30%、26.70%、26.70%、26.70%、23.30%、86.70%、23.30%、26.70%降低明显,(RR<1,Pc<0.05)。结论:云南肺癌易感性可能与HLA-A~*02的频率(90%)具有相关性;而HLA-A~*31、HLA-A~*33、HLA-B~*52、HLA-B~*27、HLA-DRB1-~*03、HLA-DRB3~*01、HLA-DQB1~*02及HLA-DQB1~*06在肺癌患者中的频率较低,在云南肺癌发病中可能具有遗传拮抗作用。Objective：To evaluate and observe the human leukocyte antigens HLA-A,B,DRB1,DQB1 alleles＆#39; frequency in Yunnan human lung cancer,and explore the relationship between the Yunnan human lung cancer mobility and the human leukocyte antigens HLA alleles.Methods：Polymerase chain reaction-sequence specific primers（PCR-SSP） method was used to analyze the HLA-A,HLA-B,HLA-DRB 1,HLA-DQB 1 alleles from 30 Yunnan lung cancer patients,compared with control group.Additionally,relative risk（RR） was calculated and analyzed,subsequently.Results：The HLA-A＊02 allele＆#39;s frequency was up to 90.0%,particularly for A＊0201,and B＊46 allele＆#39;s frequency was 40.0%,DRB1＊15 was 40% in the lung cancer group.On the contrary,HLA-A＊02 in control group was 43.30%.Meanwhile,B＊46 was 0%,DRB1＊15 was 10% in control group.The data obtained above were indicated that the frequencies in lung cancer group was significantly higher compared with those in the control group,RR〈1,pc〈0.05.Contrarily,in the lung cancer group,the frequency of HLA-A＊31 was 3.30%,A＊33 was 6.70%,B＊27 was 3.30%,B＊52 was 90.0%,DRB1＊03 was 0%,DRB3＊01 was 60.0%,DQB1＊02 was 0%,DQB1＊06 was 0%.However,the frequency of A＊31 was 23.30%,A＊33 was 26.70% in control group.Meanwhile,B＊27 in control group was 26.60%,B＊52 was 26.70%.DRB1＊03 showed its frequency as 23.30%,and DRB3＊01 was indicated as 86.70% in control group.DQB 1＊02 was detected as 23.30%,DQB 1＊06 was 26.70% in control group.The data indicated above showed its significantly lower tendency compared with control group,RR〈1,pc〈0.05.Conclusions：The HLA-A＊02 allele,up to 90.0%,was showing its higher frequency in Yunnan lung cancer group,indicating its genetically predisposed possibility.Contrarily,HLA-A＊31,HLA-A＊33,HLA-B＊27,HLA-B＊52,as well as HLA-DRB1＊03,HLA-DRB3＊01,HLA-DQB1＊02 and HLA-DQB1＊06,which were lower in cancer group,might play its potential protective roles in lung cancer.

关 键 词：云南肺癌 基因频率相关性 人类白细胞抗原 相关性研究 等位基因 

分 类 号：R734.2[医药卫生—肿瘤]