大孔吸附树脂富集玛咖总黄酮的工艺研究  

Purification of total flavonoids from Maca by macroporous resin

作　　者：李玉娟[1] 王伟[1] 陈燕文[1] 郑梦梦[1] 许方雪 席晓志 韩春超[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国食品添加剂》2017年第7期155-159,共5页China Food Additives

摘　　要：目的:优化玛咖总黄酮树脂纯化工艺参数。方法:以玛咖总黄酮纯度为指标,通过静态——动态吸附试验,确定树脂纯化工艺的最佳参数。结果:AB-8大孔树脂分离纯化黄酮的最佳工艺参数为:以质量浓度为0.84g/m L的药液进行上柱,上样液p H为:3~4,洗脱流速为:1m L/min,除杂溶剂为:以蒸馏水冲至Molish反应呈阴性和4BV的20%乙醇,洗脱溶剂为:收集70%乙醇洗脱液2~4BV,总黄酮纯度可达15.73%。结论:工艺稳定、简单、重现性好,可用于玛咖总黄酮的富集。Objective ： To investigate the purification technology of total flavonoids from Maca by macroporous resin. Methods ： The optimum process parameters of resin were chosen with static and dynamic adsorption and desorption experiments and used the purity of flavonoids as the index. Results： The optimal technology parameters of AB-8 resin were as follows, pH 3 to 4, flow velocity of loading sample lmL/min, distillated water washing till Molish reaction of negative, 4BV 20% ethanol, 70% ethanol elution 2 - 4BV. Under the above conditions, the purity of total flavonoids was up to 15.73%. Conclusion ： This purification technology was stable, simple and with good repeatability. It could effectively enrich Maca total flavonoids.

关 键 词：玛咖 黄酮 大孔树脂 纯化 

分 类 号：TS202.3[轻工技术与工程—食品科学]