高效液相色谱法同时测定食品中纳他霉素和富马酸二甲酯的含量  

Simultaneous determination of natamycin and dimethyl fumarate in foods by high performance liquid chromatography

作　　者：官咏仪[1] 邱志超 金梦[1] 宋阳[1] 张美金[1] 陈毓芳[1] 谢玉珊[1] GUAN Yong-Yi QIU Zhi-Chao JIN Meng SONG Yang ZHANG Mei-Jin CHEN Yu-FangI XIE Yu-Shan(Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China)

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广州510623 [2]广州质量监督检测研究院,广州511447

出　　处：《食品安全质量检测学报》2017年第6期2246-2250,共5页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法同时测定食品中纳他霉素、富马酸二甲酯的分析方法。方法样品用甲醇溶解提取、超纯水定容,Agilent TC-C_(18)柱色谱分离,采用高效液相色谱-光电二极管阵列检测器进行检测,并以峰面积外标法定量。结果在4种基质、4个添加水平下,纳他霉素的平均回收率在88.1%~97.7%之间,相对标准偏差为2.47%~5.72%,检出限为0.10 mg/kg;富马酸二甲酯的平均回收率在84.5%~104%之间,相对标准偏差为1.21%~5.28%,检出限为0.05 mg/kg。结论该方法具有操作简便、快速、准确的特点,适用于食品中纳他霉素、富马酸二甲酯的含量分析。Objective To establish a method for simultaneous determination ofnatamycin and dimethyl fumarate （DMF） in foods by high performance liquid chromatography （HPLC）. Methods Samples were extracted by methanol and water, separated by Agilent TC-C18 column, detected by HPLC with photo-diode array detector （PDA） and quantified by the external standard method. Results At 4 different spiked levels in 4 kinds of food, the average recoveries of natamycin were between 88.1%-97.7%, with the RSD of 2.47%-5.72%. The average recoveries of dimethyl fumarate were between 84.5%-104%, with the RSD of 1.21%-5.28%. Detection limits of natamycin and DMF were 0.10 mg/kg and 0.05 mg/kg, respectively. Conclusion This method is simple, fast and accurate, which can he applied to the analysis of the content of natamycin and DMF in foods.

关 键 词：高效液相色谱法 纳他霉素 富马酸二甲酯 

分 类 号：O657.72[理学—分析化学]