小RNA对细菌生物膜形成的调控研究进展  

Advance in research on regulation of sRNAs in bacterial biofilm formation

作　　者：高肖芳 刘子中[2] 李文亮[2] 杨瑞馥[2] 韩延平[2] 

机构地区：[1]安徽医科大学,合肥230032 [2]军事医学科学院微生物流行病研究所、病原微生物生物安全国家重点实验室,北京100071

出　　处：《军事医学》2017年第6期530-533,542,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31430006);国家重点基础研究发展计划(973计划)资助项目(2014CB744405)

摘　　要：小RNA在细菌的生长过程中发挥重要调控作用,在感受到外界信号,如营养物浓度、温度、pH值和渗透压的改变时,小RNA可通过影响靶标基因的表达以适应环境的变化。细菌由浮游状态向固体附着生长状态的转变过程中自身会产生一种细胞外基质,即生物膜,其主要由蛋白质、多糖和DNA组成。最新研究表明,小RNA在调控细菌生物膜形成的系统中发挥着重要的作用。小RNA通过与靶标mRNA碱基配对或与调节蛋白相互作用,在细菌生物膜形成中发挥调控作用。该文综述了小RNA对细菌生物膜形成的调控机制和途径,阐述了3种经典的小RNA调控细菌生物膜形成的模型,以及小RNA调控对细菌生物膜形成的研究现状和发展方向。Small RNAs（sRNAs）play a significant role in the regulation of bacterial growth.When sensing certain environmental cues such as fluctuation of nutrient concentration,temperature,pH,and osmolarity,sRNAs can influence the expression of target genes.The formation of biofilms is initiated by bacteria transitioning from the planktonic to the surface-associated mode of growth,which is a self-produced extracellular matrix composed of proteins,polysaccharides,and DNA.Recent evidences have shown that small RNA plays an important role in the regulation of bacterial biofilm formation.sRNAs have key roles in biofilm formation process by base pairing with target mRNAs or interaction with modulating proteins.This review discussed the regulation mechanism and pathway of sRNAs in bacterial biofilms formation,and summarized three classical regulatory models of sRNAs in bacterial biofilms formation,this review also gives the research status and development direction of sRNAs in bacterial biofilms formation.

关 键 词：小RNA 生物膜 C-DI-GMP 

分 类 号：R378[医药卫生—病原生物学]