复合酶法提取矮地茶总酚酸的工艺研究  

Extraction Technology of Total Phenol from Ardisiae japonicae by Compound Enzymatic Hydrolysis

作　　者：廖春燕[1,2] 黄禧擎 符志珍 黄瑶[1,2] 

机构地区：[1]广西科技大学生物与化学工程学院,柳州545006 [2]广西科技大学广西糖资源绿色加工重点实验室,柳州545006

出　　处：《食品工业》2017年第8期99-102,共4页The Food Industry

基　　金：广西高校中青年教师基础能力提升项目(KY2016LX166);广西高校大学生创新创业训练计划项目(201510594094)

摘　　要：利用果胶酶和纤维素酶组合处理矮地茶,优化总酚酸的提取工艺。采用单因素和正交试验,研究复合酶质量比、加酶量、pH、酶解温度和酶解时间对总酚酸提取率的影响。试验结果表明最佳工艺条件为:果胶酶和纤维素酶质量比2∶1,加酶量3%,酶解时间3 h,pH 4.0,酶解温度50℃。与单酶法、热水浸提法比较,复合酶法更利于矮地茶总酚酸的提取。The total phenol was extracted from Ardisiaejaponicae by compound enzyme hydrolysis method to improve the extraction conditions. The influences of compound enzymes ratio, enzyme dose, pH, hydrolysis time and hydrolysis temperature on extraction efficiency of total phenol were investigated by single factor tests and orthogonal tests. The results showed that the best optimized process was the ratio ofpectinase to cellulose 2 ： 1, compound enzymes dosage of 3%, hydrolysis time 3 h, pH 4.0 and hydrolysis temperature 50 ℃. The yield of total phenol was higher than that of one enzyme method and hot water extraction. It provided a theoretical basis for the extraction process of flavonoids in Gynura bicolor for industrialization.

关 键 词：矮地茶 总酚酸 纤维素酶 果胶酶 

分 类 号：TQ464[化学工程—制药化工]