菠菜叶蛋白泡沫法分离工艺的优化  

Optimization on foam separation process for spinach leaf protein

作　　者：刘龙[1] 张炜[1] 陈元涛[1] 刘海彬[1] 高中超[1] 雷蕾[1] 

机构地区：[1]青海师范大学化学系,青海西宁810008

出　　处：《食品与机械》2017年第6期169-175,共7页Food and Machinery

基　　金：青海省基础研究计划项目(编号:2017-ZJ-712)

摘　　要：为有效开发利用菠菜叶蛋白资源,以菠菜叶为原料,在单因素试验基础上,以回收率和富集比为指标,选取稀释倍数、气速、温度和pH值为考察因素,根据Box-Behnken试验设计原理进行响应面试验设计优化得出泡沫法分离菠菜叶蛋白的最佳工艺条件为:稀释倍数16倍、气速260mL/min、温度40℃、pH 7.5,在该条件下,回收率和富集比分别达81.56%、14.94。研究结果表明泡沫分离技术是实现菠菜叶蛋白粗分离的一种有效方法。In order to develop and utilize spinach leaf protein resources effectively,the spinach leaves were used as raw material in this work.Based on the single factor experiments,Box-Behnken design was adopted to investigate the influence of four factors：the dilution ratio,gas velocity,temperature and pH on recovery rate and enrichment ratio of leaf protein from spinach leaves.The optimum conditions for the foam separation of spinach leaf protein were found：diluting time of 16 times,gas velocity of 260mL/min,temperature of40 ℃,pH value of 7.5.Under these conditions,the recovery and enrichment ratio reached to 81.56% and 14.94,respectively.The results show that foam separation technique is an effective method to separate leaf protein from spinach.

关 键 词：菠菜叶 蛋白 泡沫分离 回收率 富集比 

分 类 号：TS201.21[轻工技术与工程—食品科学]