乳铁蛋白及张应力对成骨细胞成骨功能影响的实验研究  

The effects of lactoferrin and tension stress on osteoblast ossification

作　　者：周泽渊[1] 杨祯瑾[1] 黄鹂[1] 王欣[1] 宿晨曦 邹淑娟[1] 

机构地区：[1]四川大学华西口腔医院正畸科,成都610000

出　　处：《现代口腔医学杂志》2017年第4期193-197,共5页Journal of Modern Stomatology

基　　金：国家自然科学基金(81470777)

摘　　要：目的探究乳铁蛋白(LF)及张应力施加对成骨细胞成骨功能的影响。方法体外培养成骨细胞,随机分为7组:加LF组,加力组,加LF+力组,加LF+ERK1/2通路抑制剂组,加力+ERK1/2通路抑制剂组,加LF+力+ERK1/2通路抑制剂组及空白对照组,采用茜素红染色检测细胞矿化能力的改变,PCR及Western Blot检测Col1、ALP、ERK1/2、P-ERK1/2分泌和蛋白表达的改变。结果 12h张应力加载下100ug/ml的LF能显著促进MC3T3-E1细胞矿化功能及Col1、ALP的m RNA和蛋白的表达,P-ERK1/2蛋白的表达也出现明显升高,而总蛋白ERK1/2未见明显变化。且加入ERK1/2通路抑制剂后,Col1、ALP、P-ERK1/2蛋白都出现显著降低。结论 LF的加入和张应力加载都可以促进MC3T3-E1细胞的成骨功能且可能有协同作用,二者的促进作用可能经过了ERK1/2信号通路的传导。Objective To investigate the influence of lactoferrin and tension stress on ossification of osteoblast. Methods Osteoblast were cultured in vitro and randomly divided into 7 groups： the group with lactoferrin, with tension stress, with lactoferrin and tension stress, with lactofetrin and inhibitors of ERK1/2 pathways, with tension stress and inhibitors of ERK1/2 pathways, with lactoferrin ,tension stress and inhibitors of ERK1/2 pathways, and control group. Observe the change of cell mineralization ability using alizarin red staining test, and the change of expression on Coil, ALP, ERK1/2 and P-ERKI/2 by using PCR and Western Blot. Results Under the tension stress for 12h, 1000ug/ml of lactoferrin could significantly promote the cell mineralization ability of MC3T3-E1 cells and the expression of Co11, ALP, mRNA and P-ERK 1/2, while ERK1/2 didn＇t change obviously. After adding inhibitors of ERK1/2 pathways, the expression of Coil, ALP and P-ERK1/2 were significantly reduced. Conclusion Lactoferrin and tension stress could promote the cell mineralization ability of MC3T3-E1 cells and they might achieve it synergistically. The promotion may be impacted through ERK1/2 pathways.

关 键 词：乳铁蛋白 张应力 成骨功能 ERK1/2 

分 类 号：R782[医药卫生—口腔医学]