结直肠癌肿瘤微环境中调节性T细胞CD39、CD73亚群及二者双阳性亚群的临床意义  

Clinical significance of CD39 CD73 subsets and both of them positive subgroups of regulatory T cells in the microenvironment of colorectal cancer

作　　者：王东亮[1] 戚宁[1] 牟冠男[1] 张月桂[2] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院,哈尔滨150081 [2]哈尔滨医科大学肿瘤研究所

出　　处：《实用肿瘤学杂志》2017年第4期329-334,共6页Practical Oncology Journal

基　　金：黑龙江省教育厅科学技术研究(面上)项目计划(12541485)

摘　　要：目的观察结直肠癌肿瘤组织微环境中浸润的调节性T细胞的临床意义,并探究其CD39、CD73及CD39,CD73双阳性亚群与肿瘤微环境中其他淋巴细胞及临床病理参数之间的关系。方法收集24例结直肠癌患者根治术后组织标本,剪碎并分离培养组织内肿瘤浸润淋巴细胞(Tumor infiltrating lymphocyte,TIL),以流式细胞术观察调节性T细胞CD39^+、CD73^+及CD39^+CD73^+亚群与患者临床病理参数之间的关联。结果结直肠癌肿瘤组织微环境中浸润的调节性T细胞CD73^+亚群,CD39^+CD73^+亚群与淋巴结转移及分化程度不良具有相关性,并且其机制与肿瘤相关性炎症密切相关。结论相比Treg中的其他亚群,CD39^+CD73^+阳性的结直肠肿瘤浸润Treg具有更为独特的生物学活性,本研究为预测结直肠癌预后提供了新思路与理论依据。Objective The objectives of this study were to investigate the clinical significance of CD39,CD73,double positive subgroups for CD39 and CD73,and other lymphocytes with clinicopathological parameters in the microenvironment of colorectal cancer.Methods Tumor infiltrating lymphocyte（TIL）was collected from 24 patients with colorectal cancer after radical resection.The expression of CD39＋,CD73＋ or CD39＋ with CD73＋ in T cells were measured by flow cytometry.The association between these subgroups and clinicopathologic parameters was analyzed.Results The CD73＋ and CD39＋ with CD73＋ subgroups were associated with lymph node metastasis and poor degree of differentiation,and this mechanism was closely related to tumor-associated inflammation.Conclusion CD39＋ with CD73＋ colorectal tumor infiltration Treg has a more unique biological activity than other Treg group.This study provides a new idea and theoretical basis for predicting the prognosis of colorectal cancer.

关 键 词：结直肠癌 肿瘤浸润的淋巴细胞 调节性T细胞 CD39 CD73 

分 类 号：R735.34[医药卫生—肿瘤]