大蒜素抑制多发性骨髓瘤侧群细胞增殖机制研究  

Mechanism of DATS Inhibiting Side Population Cell Proliferation in Multiple Myeloma

作　　者：曾俊权[1] 郑永亮[1] 刘彬[1] 练建平[1] 龚志南[2] 

机构地区：[1]井冈山大学附属医院血液肿瘤科,江西吉安343000 [2]井冈山大学附属医院科技教育科,江西吉安343000

出　　处：《中国实验血液学杂志》2017年第4期1086-1091,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金(81660678);江西省教育厅科技计划项目(GJJ150765);江西省卫生计生委科技计划项目(20165391;2016A171)

摘　　要：目的:探讨大蒜素对多发性骨髓瘤侧群细胞(side population,SP)增殖的影响及其作用机制。方法:培养RPM I-8226和NCI-H929细胞,应用Hoechst33342染色分析其侧群细胞的含量;对培养的侧群细胞,应用10μg/ml大蒜素处理,采用CCK8法分析大蒜素对侧群细胞增殖的影响,应用平板集落形成法分析大蒜素对侧群细胞集落形成能力的影响,流式细胞术分析大蒜素对侧群细胞周期的影响,Western blot法分析大蒜素对周期相关蛋白cyclin D1、cyclin E、CDK2和CDK4的表达。结果:在RPM I 8226和NCI-H929中均存在有侧群细胞,其所占比例分别为(3.17±0.98)%和(2.65±0.61)%。大蒜素能够呈时间依赖性抑制侧群细胞的增殖并显著抑制集落形成;大蒜素能够抑制周期相关蛋白cyclin D1、cyclin E、CDK2和CDK4的表达并诱导细胞发生G1/S周期阻滞。结论:大蒜素可以抑制多发性骨髓瘤侧群细胞增殖和集落的形成,并可能通过下调细胞周期相关蛋白的表达诱导细胞发生G1/S阻滞。Objective： To study the effect of diallyl thiosulfinate（ DATS） on the proliferation of side population（ SP） cells in multiple myeloma（ MM） and its mechanism. Methods： RPMI-8226 and NCI-H929 cells were cultured,and the level of SP cells was detected by Hoechst33342 staining. The SP cells were cultured and treated with 10 μg/ml DATS,the CCK8 assay was carried out to examine the effect of DATS on the proliferation ability in SP cells,and plate colony-forming test was used to examine the colony-forming ability,the flow cytometry assay was carried out to examine the cell cycle,Western blot assay was used to examine the expression of cyclin D1,cyclin E,CDK2 and CDK4.Results： SP cells were detected in RPMI-8226 and NCI-H929 cells with a proportion of 3. 17 ± 0. 98 and 2. 65 ± 0. 61,respectively. DATS treatment could significantly inhibit the SP cells survival in a time-dependent manner,and also could significantly inhibit the colony forming. In addition,DATS treatment could significantly induce the G1/S arrest and suppress the expression of cyclin D1,cyclin E,CDK2 and CDK4. Conclusion： DATS can inhibit the proliferation and colony-forming of SP cells in multiple myeloma,and induce the G1/S arrest that may be carried out via suppressing the expression of cyclin D1,cyclin E,CDK2 and CDK4.

关 键 词：多发性骨髓瘤 大蒜素 侧群细胞 细胞增殖 集落形成 细胞周期 

分 类 号：R733.3[医药卫生—肿瘤]